The alternative sigma factor σ B plays a crucial role in adaptive strategies of Clostridium difficile during gut infection

Autor: Kint, Nicolas, Janoir, Claire, Monot, Marc, Hoys, Sandra, Soutourina, Olga, Dupuy, Bruno, Martin-Verstraete, Isabelle
Přispěvatelé: Pathogénèse des Bactéries Anaérobies / Pathogenesis of Bacterial Anaerobes (PBA (U-Pasteur_6)), Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7), Université Paris Diderot - Paris 7 (UPD7), Bactéries, Pathogènes et Santé (UBaPS), Faculté de Pharmacie, Université Paris-Sud - Paris 11 (UP11)-Université Paris-Sud - Paris 11 (UP11), This work was funded by the Institut Pasteur and the University Paris 7., We are thankful to Thomas Dubois and Al Claiborne for helpful discussions and to Jovana Mihajlovic and Jean-Marc Ghigo for their help with oxygen assays., Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7), Université Paris-Sud - Paris 11 (UP11)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11)-Université Paris-Saclay
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Environmental Microbiology
Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2017, Special Issue on Pathogen and Antibiotic Resistance Ecology 19 (5), pp.1933-1958. ⟨10.1111/1462-2920.13696⟩
Environmental Microbiology, 2017, Special Issue on Pathogen and Antibiotic Resistance Ecology 19 (5), pp.1933-1958. ⟨10.1111/1462-2920.13696⟩
Environmental Microbiology, Wiley-Blackwell, 2017, Special Issue on Pathogen and Antibiotic Resistance Ecology 19 (5), pp.1933-1958. ⟨10.1111/1462-2920.13696⟩
ISSN: 1462-2912
1462-2920
DOI: 10.1111/1462-2920.13696⟩
Popis: International audience; Clostridium difficile is a major cause of diarrhoea associated with antibiotherapy. Exposed to stresses in the gut, C. difficile can survive by inducing protection, detoxification and repair systems. In several firmicutes, most of these systems are controlled by the general stress response involving σ(B) . In this work, we studied the role of σ(B) in the physiopathology of C. difficile. We showed that the survival of the sigB mutant during the stationary phase was reduced. Using a transcriptome analysis, we showed that σ(B) controls the expression of ∼25% of genes including genes involved in sporulation, metabolism, cell surface biogenesis and the management of stresses. By contrast, σ(B) does not control toxin gene expression. In agreement with the up-regulation of sporulation genes, the sporulation efficiency is higher in the sigB mutant than in the wild-type strain. sigB inactivation also led to increased sensitivity to acidification, cationic antimicrobial peptides, nitric oxide and ROS. In addition, we showed for the first time that σ(B) also plays a crucial role in oxygen tolerance in this strict anaerobe. Finally, we demonstrated that the fitness of colonisation by the sigB mutant is greatly affected in a dixenic mouse model of colonisation when compared to the wild-type strain.
Databáze: OpenAIRE
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