Bacteriocin production by lactic acid bacteria of Rioja red wines
| Autor: | Navarro, L., Zarazaga, M., Sáenz, J., Ruiz-Larrea, F., Torres, C. |
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| Rok vydání: | 2000 |
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| Zdroj: | RIUR. Repositorio Institucional de la Universidad de La Rioja instname RIUR: Repositorio Institucional de la Universidad de La Rioja Universidad de La Rioja (UR) |
| Popis: | Forty-two lactic acid bacteria (LAB) of the genera Lactobacillus (32), Leuconostoc (6), Pediococcus (3) and Lactococcus (1), isolated from Rioja red Wines, were tested for antimicrobial activity. All these strains, as well as 18 Leuconostoc oenos and 19 yeast strains were used as indicators. Only nine strains showed antimicrobial activity, and all were of the species Lactobacillus plantarum, which constitutes the predominant micro flora in Rioja red wines after alcoholic fermentation. Lact. plantarum strain J-51 showed the widest range of action, inhibiting the growth of 31 strains of the four studied LAB genera. Lact. plantarum J-51 antimicrobial activity was lost after treatment with proteases, suggesting a proteinaceous nature for this activity. It was found to be stable between pH 3 and 9 and under strong heating conditions (100 °C for 60 min). Polymerase chain reaction (PCR) of Lact. plantarum J-51 genome the presence analysis revealed of the plnA gene that encodes the plantaricin precursor PlnA. A 366-bp fragment was sequenced and showed 95% identity with pln locus of Lact. plantarum C-11. The deduced precursor peptide sequence showed one mutation (Gly7 to Ser7) at the double glycine leader peptide, and the three putative 26-, 23- and 22-residue active peptides remain identical to those of Lact. plantarum C-11. Therefore, antimicrobial peptides constitute a potent adaptation advantage for those strains that dominate in a medium such as wine, and can play an important role in the ecology of wine microflora. |
| Databáze: | OpenAIRE |
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