Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys

Autor: Li, Sl, Feichtinger, H, Kaaya, E, Migliorini, Paola, Putkonen, P, Biberfeld, G, Middeldorp, Jm, Biberfeld, P, Ernberg, I.
Přispěvatelé: Pathology, CCA - Cancer immunology, CCA - Target Discovery & Preclinial Therapy Development, CCA - Biomarkers, CCA - Evaluation of Cancer Care, AII - Infectious diseases, AII - Cancer immunology
Jazyk: angličtina
Rok vydání: 1993
Předmět:
Zdroj: International Journal of Cancer, 55(4), 609-15. Wiley-Liss Inc.
Li, S L, Feichtinger, H, Kaaya, E, Migliorini, P, Putkonen, P, Biberfeld, G, Middeldorp, J M, Biberfeld, P & Ernberg, I 1993, ' Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys ', International Journal of Cancer, vol. 55, no. 4, pp. 609-15 .
ISSN: 0020-7136
Popis: Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.
Databáze: OpenAIRE
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