Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes
| Autor: | Juárez Ortega, Paloma, Presa Castro, Silvia, Espi, Joaquin, Pineda Chaza, Benito José, Antón Martínez, María Teresa, Moreno Ferrero, Vicente, Buesa, Javier, Granell Richart, Antonio, Orzáez Calatayud, Diego Vicente |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Rotavirus
B-Subunit Passive immunization fungi Monoclonal-antibodies food and beverages Carotenoid biosynthesis Expression Identity preservation Plants Tomato Fruits Anthocyanins GENETICA Capsid proteins Plant synthetic biology BIOQUIMICA Y BIOLOGIA MOLECULAR Secretory antibodies Antigens Fusion protein IgA |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Popis: | El pdf del artículo es la versión pre-print. Edible fruits are inexpensive biofactories for human health-promoting molecules that can be ingested as crude extracts or partially purified formulations. We show here the production of a model human antibody for passive protection against the enteric pathogen rotavirus in transgenically labelled tomato fruits. Transgenic tomato plants expressing a recombinant human immunoglobulin A (hIgA_2A1) selected against the VP8* peptide of rotavirus SA11 strain were obtained. The amount of hIgA_2A1 protein reached 3.6 +/- 0.8% of the total soluble protein in the fruit of the transformed plants. Minimally processed fruit-derived products suitable for oral intake showed anti-VP8* binding activity and strongly inhibited virus infection in an in vitro virus neutralization assay. In order to make tomatoes expressing hIgA_2A1 easily distinguishable from wild-type tomatoes, lines expressing hIgA_2A1 transgenes were sexually crossed with a transgenic tomato line expressing the genes encoding Antirrhinum majus Rosea1 and Delila transcription factors, which confer purple colour to the fruit. Consequently, transgenically labelled purple tomato fruits expressing hIgA_2A1 have been developed. The resulting purple-coloured extracts from these fruits contain high levels of recombinant anti-rotavirus neutralizing human IgA in combination with increased amounts of health-promoting anthocyanins. This work was supported by the Spanish Ministry of Science, Technology and Innovation: Grants BIO2008-03434, AGL2009-13388-C03-01 and BIO2010-15384 and P. Juarez FPU fellowship. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|