Identification of IL-10 and TGF-beta transcripts involved in the inhibition of T-lymphocyte proliferation during cell contact with human mesenchymal stem cells
| Autor: | Nasef, Aisha, Chapel, Alain, Mazurier, Christelle, Bouchet, Sandrine, Lopez, Manuel, Mathieu, Noelle, Sensebé, Luc, Zhang, Yizhuo, Gorin, Norbert-Claude, Thierry, Dominique, Fouillard, Loïc |
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| Přispěvatelé: | CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Saas, Philippe |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
MESH: Cell Culture Techniques
MESH: Interleukin-10 MESH: Humans [SDV.IMM] Life Sciences [q-bio]/Immunology MESH: Immunosuppression MESH: Gene Expression Regulation MESH: Coculture Techniques MESH: Mesenchymal Stem Cells MESH: T-Lymphocytes MESH: Cell Proliferation MESH: Cell Communication MESH: Cell Adhesion Molecules [SDV.IMM]Life Sciences [q-bio]/Immunology MESH: Chemokine CXCL12 MESH: Transforming Growth Factor beta MESH: Chemokines CXC MESH: Cells Cultured |
| Zdroj: | Gene expression Gene expression, 2007, 13 (4-5), pp.217-26 |
| Popis: | International audience; Mesenchymal stem cells (MSC) inhibit the response of allogeneic T lymphocytes in culture. Because the mechanisms of this effect may differ according to the existence of cell contact, we investigated the differences in gene expression of inhibitory molecules during MSC-T lymphocyte coculture when cell contact does and does not occur. Human MSC and T lymphocytes were cultured together in standard and transwell cultures. MSC gene expression was analyzed by semiquantitative real-time RT-PCR. MSC elicited a high dose-dependent inhibition of T lymphocytes in cultures with cell contact, but inhibition occurred even without cell contact. In both cases, we observed significant upregulation of IDO, LIF, and HLA-G, along with downregulation of HGF and SDF1. In cultures with cell contact, IL-10 and TGF-beta transcripts were expressed in a significantly higher level than in cultures without this contact. Furthermore, in the latter, the increased inhibition of T-cell proliferation was positively correlated with IDO gene expression and negatively correlated with SDF1 gene expression. MSC appear to induce T-cell tolerance by two distinct mechanisms. The first of these, which does not require cell contact, induces expression of the tolerogenic genes IDO, LIF, and HLA-G. The second mechanism, which is contact dependent, modulates IL-10 and TGF-beta gene expression. These two mechanisms probably play separate roles in MSC-induced tolerance in allogeneic hematopoietic stem cell transplantation. |
| Databáze: | OpenAIRE |
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