Ligase detection reaction and universal array as a tool to detect grapevine infecting phytoplasmas

Autor: Frosini, A., Casati, P., Bianco, P. A., Bordoni, R., Consolandi, C., Castiglioni, B., Mezzelani, A., ermanno rizzi, Battaglia, C., Belli, G., Rossi Bernardi, L., Bellis, G.
Rok vydání: 2002
Předmět:
Zdroj: Minerva biotecnologica (Testo stamp.) 14 (2002): 265–267.
info:cnr-pdr/source/autori:Frosini A., Casati P., Bianco P. A., Bordoni R., Consolandi C., Castiglioni B., Mezzelani A., Rizzi E., Battaglia C., Belli G., Rossi Bernardi L., De Bellis G./titolo:Ligase detection reaction and universal array as a tool to detect grapevine infecting phytoplasmas/doi:/rivista:Minerva biotecnologica (Testo stamp.)/anno:2002/pagina_da:265/pagina_a:267/intervallo_pagine:265–267/volume:14
Scopus-Elsevier
Popis: Phytoplasmas are unculturable wall less bacteria involved in many plant diseases; in particular Flavescence dorée represents a severe threat to viticulture, because of its epidemic behavior in field. Here we present the application of the LDR technique, associated with hybridisation on universal arrays, as a quick and reliable tool for plant quarantine procedures and mandatory measures, in order to distinguish between different phytoplasmas’ induced infections, compared to classical protocols in use, particularly PCR - RFLP assays.
Databáze: OpenAIRE