In vitro dynamic model applied to interrogate the impact of pasteurization on preterm human milk digestion: comparison against in vivo data

Autor: DE OLIVEIRA, Samira, Bourlieu-Lacanal, Claire, Ménard, Olivia, Bellanger, Amandine, Moustiés, Célia, Carrière, F., Dirson, Emelyne, Le Gouar, Yann, Rousseau, Florence, Pladys, Patrick, Dupont, Didier, Deglaire, Amélie
Přispěvatelé: Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Service de Pédiatrie, Centre Hospitalier Universitaire [Rennes], Enzymologie interfaciale et de physiologie de la lipolyse (EIPL), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Lactarium - Unité Nutrition et Diététique Infantile, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: 3.International Conference on Food Structures, Digestion and Health
3.International Conference on Food Structures, Digestion and Health, Oct 2015, Wellington, New Zealand. 2015
Popis: This work was integrated in the COST action FA1005 INFOGEST, and the researchers associated are acknowledged for contribution to the discussion. The authors warmly thank the volunteer mothers and the colleagues from the bank milk of the Rennes University Hospital Center. The author SCDO acknowledges the PhD scholarship from CNPq BrazilThis work was integrated in the COST action FA1005 INFOGEST, and theresearchers associated are acknowledged for contribution to the discussion. The authors warmly thankthe volunteer mothers and the colleagues from the bank milk of the Rennes University HospitalCenter. The author SCDO acknowledges the PhD scholarship from CNPq Brazil; Understanding the behavior of human milk in the newborn gastrointestinal tract is a key step in developing substitutes with optimized health benefits. However, ethical and technical reasons limit the possibility of in vivo trials. Consequently, it is important to develop relevant in vitro models. The aim of this work was to compare a dynamic in vitro digestion system developed by the French National Institute for Agricultural Research1 against in vivo data from raw or pasteurized human milk gastric digestion.Supported by an exhaustive literature review2, the dynamic digester parameters were set in terms of types and amounts of enzymes, secretions, pH decrease and emptying rate to mimic as closely as possible the gastric digestive conditions of preterm newborns. Raw or pasteurized (62.5°C, 30 min) pooled preterm human milks (n=5 donors) were digested in triplicate3. In parallel, in vivo study was conducted on hospitalized preterm newborns at Rennes Hospital (NCT02112331, n=12)4. Gastric digesta were sampled regularly. Structural changes were evaluated by confocal microscopy and laser light scattering. Lipolysis and proteolysis kinetics were monitored by SDS-Page, thin-layer and gas chromatography methods. Likewise, gastric volume, pH and emptying rates were compared. During both in vitro and in vivo gastric digestions, pasteurization did not affect the kinetics and final degree of lipolysis and proteolysis, but impacted on emulsion disintegration, protein aggregation and the persistence of native fat globule structure. Results showed the rapid hydrolysis of caseins and a resistance of alpha-lactalbumin in vitro and in vivo. The relatively limited extent of lipolysis by the gastric lipase was confirmed in vitro and in vivo, with a lipolysis degree ranging from 7 to 19% at 90 min.Although curves of pH decrease and emptying rates can be improved to adjust closer to in vivo data, kinetics of hydrolysis and disintegration of human milk showed that thedynamic system of in vitro newborn digestion proposed here is a relevant tool to study gastric digestive kinetics. Our model will be useful to the scientist community and food manufacturers who focus on neonatal gastric digestion and infant formulas optimization.
Databáze: OpenAIRE
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