| Autor: |
Yokouchi, Hiroshi, Yamazaki, Koichi, Asahina, Hajime, Shigemura, Masahiko, Moriyama, Takanori, Takaoka, Kazuo, Moriya, Jun, Itoh, Tomoo, Kinoshita, Ichiro, Dosaka-Akita, Hirotoshi, Tsutsumi, Yutaka, Masaharu Nishimura |
| Jazyk: |
angličtina |
| Rok vydání: |
2006 |
| Předmět: |
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| Zdroj: |
ResearcherID |
| Popis: |
Background: Few studies have successfully established an amylase-producing lung cancer cell line or have examined its cytological, biochemical and biological features. Materials and Methods: Cancer cells isolated from pleural effusion using a gradient method were cultivated. Results: Amylase production from the newly established cell line was confirmed by positive staining for α-amylase and increased amylase levels in culture supernatant. Electron microscopy revealed zymogen granule-like structures. Sialylation of salivary-type amylase was confirmed directly from the cell line by examining neuraminidase sensitivity and amylase elution profile under high-performance liquid chromatography. EGFR or KRAS mutation was not found. Conclusions: This cell line offers a useful tool for analyzing the pathogenesis and pathophysiology of amylase-producing lung cancers. Moreover, it might be useful for probing metastasis and invasiveness of lung cancer cells and for developing an early diagnostic method based on sialylated salivary-amylase production. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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