| Autor: |
Elitas, M., Valero, A., Thomas Braschler, Dhar, N., Mckinney, J. D., Renaud, P. |
| Předmět: |
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| Zdroj: |
Scopus-Elsevier |
| Popis: |
Exposure of a bacterial culture to an antibiotic or other lethal stress typically results in the generation of sub-populations of live cells ("persisters") and dead cells, and, in some cases, viable but non-culturable cells. Studies aimed at understanding the mechanisms of antibiotic killing and bacterial persistence will require the purification and analysis of each of these sub-populations in isolation. This paper reports a method based on continuous-flow dielectrophoresis to separate live and dead cells from antibiotic-treated bacterial cultures. Our experiments were carried out on Mycobacterium smegmatis, a model organism that is closely related to the etiological agent of human tuberculosis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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