| Popis: |
Objectives of this study were to determine effects of time relative to calving and health status on blood metabolite concentrations after glucose tolerance test (GTT) and to investigate on the severity and on the distribuition of periparturient fatty change liver (FCL). Metabolic profiles were performed on plasma samples collected from 40 cows selected from 2 dairy farms. GTT and Blood sampling was carried out 7±5 d before and 7±5 d after the calving. Glucose was administrated intravenously in a 50% solution at a dose of 0,25 g/Kg. Blood samples were collected before (T0) and after the beginning of the test at different times (T2, T5, T10, T15, T20, T30, T40, T60, T80, T100, T120, T140, T160). Beta-Hydroxybutyrate (BHB), Non-Esterified Fatty Acids (NEFA), Glucose(Glu), Insuline (Ins), Aspartate Aminotransferase (AST-GOT), Alanine Transaminase (ALT-GTP), total proteins (TP), globulins(Gb), albumins(Alb) and Urea were analized. Histologic examinations by light microscopy and classifications of samples according to the severity of FCL and Inflammatory infiltrations were done. The collected data were statistically analyzed by the analysis of variance (ANOVA), using SIGMA STAT 3.7 to evaluate the effect of the period (pre-partum and post-partum) and the farm (farm A and farm B) on the hematic, clinical and productive parameters. Pearson’s coefficients were also calculated between the collected data; finally the AUC (Area Under Curve) of glucose, insulin, NEFA and BHB were calculated. The result proved that in the post-partum period cows show insulin resistance that causes a reduction of the levels of glucose and insulin (P |
