B cell OX40L supports T follicular helper cell development and contributes to SLE pathogenesis
Autor: | Cortini, Andrea, Ellinghaus, Ursula, Malik, Talat H., Cunninghame Graham, Deborah S., Botto, Marina, Vyse, Timothy James |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
EXPRESSION
autoantibodies Autoimmunity OX40 Ligand SUSCEPTIBILITY 1117 Public Health and Health Services LIGAND INTERACTION Mice systemic lupus erythematosus Rheumatology Animals Lupus Erythematosus Systemic SYSTEMIC-LUPUS-ERYTHEMATOSUS Mice Knockout B cells B-Lymphocytes Science & Technology Membrane Glycoproteins CHRONIC GRAFT 1103 Clinical Sciences ASSOCIATION T-Lymphocytes Helper-Inducer OX40L CD4 Arthritis & Rheumatology DIFFERENTIATION 1107 Immunology CHEMOKINE RECEPTOR Tumor Necrosis Factors T follicular helper cells Life Sciences & Biomedicine DENDRITIC CELL |
Zdroj: | Cortini, A, Ellinghaus, U, Malik, T H, Cunninghame Graham, D S, Botto, M & Vyse, T J 2017, ' B cell OX40L supports T follicular helper cell development and contributes to SLE pathogenesis ', Annals of the rheumatic diseases, vol. 76, no. 12, pp. 2095-2103 . https://doi.org/10.1136/annrheumdis-2017-211499 |
Popis: | OBJECTIVES: TNFSF4 (encodes OX40L) is a susceptibility locus for systemic lupus erythematosus (SLE). Risk alleles increase TNFSF4 expression in cell lines, but the mechanism linking this effect to disease is unclear, and the OX40L-expressing cell types mediating the risk are not clearly established. Blockade of OX40L has been demonstrated to reduce disease severity in several models of autoimmunity, but not in SLE. We sought to investigate its potential therapeutic role in lupus.METHODS: We used a conditional knockout mouse system to investigate the function of OX40L on B and T lymphocytes in systemic autoimmunity.RESULTS: Physiologically, OX40L on both B and T cells contributed to the humoral immune response, but B cell OX40L supported the secondary humoral response and antibody affinity maturation. Our data also indicated that loss of B cell OX40L impeded the generation of splenic T follicular helper cells. We further show that in two models of SLE-a spontaneous congenic model and the H2-IAbm12 graft-versus-host-induced model-loss of B cell OX40L ameliorates the autoimmune phenotype. This improvement was, in each case, accompanied by a decline in T follicular helper cell numbers. Importantly, the germline knockout did not exhibit a markedly different phenotype from the B cell knockout in these models.CONCLUSIONS: These findings contribute to a model in which genetically determined increased OX40L expression promotes human SLE by several mechanisms, contingent on its cellular expression. The improvement in pathology in two models of systemic autoimmunity indicates that OX40L is an excellent therapeutic target in SLE. |
Databáze: | OpenAIRE |
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