Molecular techniques for the study and diagnosis of parasite infection
| Autor: | Tavares, R. G., Rodrigo Staggemeier, Borges, A. L. P., Rodrigues, M. T., Castelan, L. A., Vasconcelos, J., Anschau, M. E., Spalding, S. M. |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Journal of Venomous Animals and Toxins including Tropical Diseases v.17 n.3 2011 The Journal of venomous animals and toxins including tropical diseases Universidade Estadual Paulista (UNESP) instacron:UNESP Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 17, Issue: 3, Pages: 239-248, Published: 2011 Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS Scopus-Elsevier |
| Popis: | In parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites. Currently, molecular biology techniques are increasingly used to diagnose parasite structures in order to enhance the identification and characterization of parasites. The objective of the present study was to review the main current and new diagnostic techniques for confirmation of parasite infections, namely: polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), Luminex xMAP, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP), in addition to microsatellites. Molecular assays have comprehensively assisted in the diagnosis, treatment and epidemiological studies of parasitic diseases that affect people worldwide, helping to control parasitic disease mortality. |
| Databáze: | OpenAIRE |
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