Stylin-1: a putative receptor of Cauliflower mosaic virus
| Autor: | WEBSTER, Craig, Blanc, Stéphane, Uzest, Marilyne |
|---|---|
| Přispěvatelé: | Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), ProdInra, Archive Ouverte, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de Recherche Agronomique (INRA). UMR Biologie et Génétique des Interactions Plante-Parasite (0385). |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | 16. Rencontres de Virologie Végétale (RVV 2017) 16. Rencontres de Virologie Végétale (RVV 2017), Jan 2017, Aussois, France 16. Rencontres de Virologie Végétale (RVV 2017), Institut National de Recherche Agronomique (INRA). UMR Biologie et Génétique des Interactions Plante-Parasite (0385)., Jan 2017, Aussois, France |
| Popis: | BGPI : équipe 2; The receptor of Cauliflower mosaic virus (CaMV) has been shown previously (Uzest et al., 2009) to be a surface exposed cuticular protein (CuP) occurring at the tip of maxillary stylets along a discrete organ called the acrostyle. However, while thelocation has been determined, the identity of this receptor has not been identified despite a plethora of attempts. Additionallyit is presumed that the receptors of other non-persistent viruses, including the Potyvirsues and Cucumber mosaic virus arelocated at, or close to, the acrostyle, hence the great interest in this region and its involvement in plant-insect and virusinsectinteractions. Here we identify for the first time a CuP which is present at the surface of the acrostyle of Acyrthosiphonpisum, making it the first protein specifically identified within the stylets of aphids and for which we propose the name stylin-1. Additionally we determined that stylin-1 collocates with the P2 helper protein of CaMV at the surface of the acrostyle andis conserved within the stylets of several important vectors of non-persistent viruses including Aphis gossypii, Brevycorniaebrassicae and Myzus persicae. Competition/blocking of the acrostyle, by sequentially labelling stylets with the P2 helperprotein and stylin-1 antibody, were used to determine the interaction of the two on dissected stylets. In these experimentsP2 reduced significantly the amount of subsequent labelling of the stylin-1 antibody indicating competition for the samemolecule. Hence, we propose that stylin-1 may be the first receptor of a non-persistent virus found. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|