MicroRNAs-424 and 206 are potential prognostic markers in spinal onset amyotrophic lateral sclerosis
| Autor: | Andrade, Helen Maia Tavares de, 1979, Albuquerque, Milena de, 1982, Avansini, Simoni Helena, 1980, Dogini, Danyella Barbosa, Rocha, Cristiane de Souza, 1978, Nucci, Anamarli, 1947, Carvalho, Benilton de Sá, 1979, Lopes-Cendes, Íscia Teresinha, 1964, Franca Junior, Marcondes Cavalcante, 1976 |
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| Přispěvatelé: | UNIVERSIDADE ESTADUAL DE CAMPINAS |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Repositório da Produção Científica e Intelectual da Unicamp Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP Repositório Institucional da Unicamp |
| Popis: | Skeletal muscle microRNAs (miRNAs) are potential candidate biomarkers for amyotrophic lateral sclerosis (ALS) that deserve further investigation. To identify miRNAs abnormally expressed in the skeletal muscle and plasma of patients with ALS, and to correlate them with parameters of disease progression. Expression profile of miRNAs in muscle was evaluated using an array platform. Subsequently we assessed the plasmatic expression of candidate miRNAs in a set of 39 patients/39 controls. We employed generalized estimating equations to investigate correlations with clinical data. We identified 11 miRNAs differentially expressed in the muscle of ALS patients; of these, miR424, miR-214 and miR-206 were validated by qPCR in muscle samples. In plasma, we found only miR-424 and miR 206 to be overexpressed. Baseline expression of miR-424 and 206 correlated with clinical deterioration over time. MiR-424 and miR-206 are potential prognostic markers for ALS CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP Fechado |
| Databáze: | OpenAIRE |
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