Telomerase in (pre)neoplastic cervical disease

Autor: Wisman, Gba, Jong, S., Meersma, Gj, Helder, Mn, Hollema, H., Vries, Ege, Nicol Keith, Zee, Agj
Přispěvatelé: Guided Treatment in Optimal Selected Cancer Patients (GUTS), Targeted Gynaecologic Oncology (TARGON)
Jazyk: angličtina
Rok vydání: 2000
Předmět:
Zdroj: Human Pathology, 31(10), 1304-1312. W B SAUNDERS CO-ELSEVIER INC
ResearcherID
ISSN: 1304-1312
0046-8177
Popis: This study was performed to determine upregulation of the human telomerase RNA component (hTR) and mRNA of the catalytic subunit of telomerase (hTERT) in (pre)malignant cervical lesions, to analyze possible intralesional heterogeneity of hTR expression, and to relate hTR and hTERT mRNA levels to telomerase activity levels and human papillomavirus (HPV) typing. hTR expression was determined by in situ hybridization (ISH) on paraffin-embedded sections, obtained from patients with cervical intraepithelial neoplasia (CIN) I-III or cervical cancer and from normal controls. hTR and hTERT mRNA expression were determined by semiquantitative rt-PCR on frozen samples from the same lesions. Data on telomerase activity and HPV were obtained from a previous study. hTR expression as determined by ISH was observed in 0 of 8 normal cervices, 1 of 14 CIN I, 15 of 28 CIN II, 21 of 30 CIN III, and 16 of 18 cervical cancer specimens. In general, hybridization patterns for hTR expression were homogeneous throughout the lesion. Frequency of hTR expression was related to grade of CIN/cervical cancer (P
Databáze: OpenAIRE
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