KNOTTED-like genes of peach: mapping and expression patterns during drupe development
| Autor: | Testone G., Verde I., Condello E., Caboni E., Vendramin E., Frugis G., Nicolodi C., Iafrate S., Galaffi F., Dettori M.T., Giannino D. |
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| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Proceedings of the 54th Italian Society of Agricultural Genetics Annual Congress, Matera, 2010 info:cnr-pdr/source/autori:Testone G., Verde I., Condello E., Caboni E., Vendramin E., Frugis G., Nicolodi C., Iafrate S., Galaffi F., Dettori M.T., Giannino D./congresso_nome:Proceedings of the 54th Italian Society of Agricultural Genetics Annual Congress/congresso_luogo:Matera/congresso_data:2010/anno:2010/pagina_da:/pagina_a:/intervallo_pagine 54° Convegno Società Italiana di Genetica Agraria, Matera, 2010 info:cnr-pdr/source/autori:Testone G., Verde I., Condello E., Caboni E., Vendramin E., Frugis G., Nicolodi C., Iafrate S., Galaffi F., Dettori M.T., Giannino D./congresso_nome:54° Convegno Società Italiana di Genetica Agraria/congresso_luogo:Matera/congresso_data:2010/anno:2010/pagina_da:/pagina_a:/intervallo_pagine |
| Popis: | Plant KNOTTED-like homeobox (KNOX) transcription factors play important roles in the development of several organs, including diverse fruit types such as tomato berries and Arabidopsis siliques. The peach genome contains ten KNOX genes (KNOPEs), six of class1, three of class 2 and one of class M, which maintain the exon-intron organisation compared to the respective Arabidopsis orthologues. Seven members were positioned on the genetic Prunus reference map by means of co-dominant markers and they were scattered on distinct linkage groups. Putative association between KNOPEs and drupe quantitative trait loci (QTLs) were inferred based on synteny/co-linearity of diverse Prunus spp. genetic maps. The expression of KNOPEs was monitored by qPCR in the drupe mesocarp of three cultivars ('Chiripa', 'Fantasia' and 'Bolero') during the S1-S4 developmental stages. As for class 1 genes, the KNOPE1 and KNOPE6 mRNA levels decreased progressively from S1 to S4, whilst those of KNOPE2, KNOPE2.1, STMlike1, STMlike2 were below the threshold levels. Interestingly, KNOPE1 and KNOPE6 genetic positions were compatible with QTLs regulating drupe size, supporting they may play roles in controlling this trait. Concerning class 2 members, in S1-S2 the KNOPE3 and 4 showed unvaried mRNA abundance, which declined later on, whereas KNOPE7 transcription diminished from S1 to S4. All the above said patterns were maintained in three cultivars. KNOPE3 showed the highest expression levels among the KNOPEs and could be detected by in situ hybridisation in late S1 drupes. The transcript occurred in the parenchyma cells and vascular bundles of mesocarp, in the nucellus of embryo sac and in the seed coat sieves. The KNOPE3 expression was previously shown to be regulated by transport sugar, hence the phloem associated expression might imply a role in fruit sugar metabolism and seed nourishment. |
| Databáze: | OpenAIRE |
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