Interest of protoplasts for lettuce breeding
| Autor: | Mazier, Marianne, Maisonneuve, Brigitte, Bellec, Yannick, Chupeau, M.C., Souche, Sylvie, Chupeau, Yves |
|---|---|
| Přispěvatelé: | Unité de recherche Génétique et amélioration des plantes (GAP), Institut National de la Recherche Agronomique (INRA), Laboratoire de biologie cellulaire et moléculaire, Unité de Pathologie Végétale (PV), Ales Lebeda, Eva Køístková, ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
virus des vegetaux
résistance aux maladies [SDV.SA]Life Sciences [q-bio]/Agricultural sciences [SDV.SA] Life Sciences [q-bio]/Agricultural sciences Lactuca sativa fungi wild Lactuca spp protoplast isolation and fusion LMV-resistance genes immunofluorescence food and beverages lmv in vitro Agricultural sciences laitue fusion de protoplastes plante légumière virus de la mosaïque de la laitue [SDV.BV]Life Sciences [q-bio]/Vegetal Biology [SDV.BV] Life Sciences [q-bio]/Vegetal Biology Sciences agricoles |
| Zdroj: | Eucarpia leafy vegetables 99 : Proceedings of the Eucarpia Meeting on Leafy Vegetables Genetics and Breeding Eucarpia leafy vegetables 99 : Proceedings of the Eucarpia Meeting on Leafy Vegetables Genetics and Breeding, Palacky University, 1999 Eucarpia leafy vegetables 99.1999, 239-244 Eucarpia leafy vegetables 99 Eucarpia leafy vegetables 99, Jun 1999, Olomouc, Czech Republic. European Association for Research on Plant Breeding, 1999 |
| Popis: | Procedures are now available for the exploitation of protoplast technology for the genetic manipu- lation of lettuce and for studies of lettuce/virus interactions. These techniques have made it possible to overcome interspecific barriers by protoplast fusion. Following the procedures we developed, we were able to obtain hybrids between Lactuca sativa and two wild Lactuca species (L. tatarica and L. perennis). Moreover, protoplast technology can overcome some of the limitations in the application of mutation techniques in crops. In this paper, we demonstrated the feasibility and the power of our in vitro techni- ques, in combination with induced mutations, for creating new variability in lettuce. Furthermore, we adapted protocols that can provide the opportunity to start studies on mechanisms associated with the different LMV-resistance genes at the cellular level. It is presently possible to obtain protoplasts from different Lactuca species containing different resistance genes. A protocol for specific staining by immu- nofluorescence of infected lettuce protoplasts is described and preliminary electroporation experiments in some Lactuca protoplasts were performed. |
| Databáze: | OpenAIRE |
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