Contamination of cattle carcasses by Escherichia coli shiga like toxin with high antimicrobials resistence

Autor: Rigobelo, Everlon Cid, Maluta, Renato Pariz, Clarissa Araujo Borges, Beraldo, Livia Gerbasi, Franco, Manoel Victor, Aparecida Maest, Lemos Sirlei, Avila, Fernando Antonio
Přispěvatelé: Universidade Estadual Paulista (Unesp)
Předmět:
Zdroj: Web of Science
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Popis: Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:25:57Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:47:17Z : No. of bitstreams: 1 2-s2.0-84856409206.pdf: 108487 bytes, checksum: 9162e166507586504bb163503e6e5ebb (MD5) Made available in DSpace on 2014-05-27T11:25:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-08-01 During processing of cattle carcasses, contamination may occurs with the transfer of microbiota of animals feaces to carcasses. This contamination many times may be by Escherichia coli carriers of virulence factor as stx and eae genes being classified as Shiga like toxin. Shiga toxin-producing Escherichia coli (STEC) is recognized wordwide as human pathogen. A survey was performed to determine the sensibility profile to several antimicrobial drugs of STEC in carcasses obtained from an abattoir in Brazil between March 2008 and August at 2009. A total of 120 STEC were isolated. All isolates were confirmed as being E. coli by their biochemical analysis and submitted to polymerase chain reaction (PCR) for detection of stx, eae and ehly genes. No strains was isolated being carriers of ehly gene. The number of isolates carriers of eae gene were 48/120. The most frequent resistance was seen against cephalothin (84.0%), streptomycin (45.0%), nalidixic acid (42.0%) and tetracycline (20.0%). Multidrug resistance (MDR) to three or more antimicrobial agents was observed in 46 (38.3%) E. coli isolates. The findings of STEC and MRD show that cattle carcasses may be a reservoir of pathogenic bacterial for the consumer public. © 2011 Academic Journals. Campus Experimental de Dracena-Universidade Estadual Paulista (UNESP), Jaboticabal Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal Departamento de Biologia Aplicada a Agropecuária Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal Departamento de Patologia Veterinária Campus Experimental de Dracena-Universidade Estadual Paulista (UNESP), Jaboticabal
Databáze: OpenAIRE
Externí odkaz: