Autor: |
Handel, AE, Sandve, GK, Disanto, G, Berlanga-Taylor, AJ, Gallone, G, Hanwell, H, Drabløs, F, Giovannoni, G, Ebers, GC, Ramagopalan, SV, Price, RN |
Jazyk: |
angličtina |
Rok vydání: |
2016 |
Předmět: |
|
DOI: |
10.1186/1741-7015-11-163 |
Popis: |
BACKGROUND: Vitamin D insufficiency has been implicated in autoimmunity. ChIP-seq experiments using immune cell lines have shown that vitamin D receptor (VDR) binding sites are enriched near regions of the genome associated with autoimmune diseases. We aimed to investigate VDR binding in primary CD4+ cells from healthy volunteers. METHODS: We extracted CD4+ cells from nine healthy volunteers. Each sample underwent VDR ChIP-seq. Our results were analyzed in relation to published ChIP-seq and RNA-seq data in the Genomic HyperBrowser. We used MEMEChIP for de novo motif discovery. 25-Hydroxyvitamin D levels were measured using liquid chromatography-tandem mass spectrometry and samples were divided into vitamin D sufficient (25(OH)D ≥75 nmol/L) and insufficient/deficient (25(OH)D |
Databáze: |
OpenAIRE |
Externí odkaz: |
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