| Popis: |
A research on the ability of protein fraction from green algae Turbinaria decurrens in territorial of South Sulawesi to inhibit the growth of Salmonella thypi and Staphylococcus aureus has been conducted. Proteins was fractionated from the crude extract using salting out method with 0-20%, 20-40%, 40-60% and 60-80% ammonium sulphate saturation. Protein was purified by a dialysis method using a selophan membrane. The protein level was determined by a Lowry method, the highest protein concentration, 5.1 mg/mL was found in the 0-20% fraction of the green algae. Antibacterial activity test was performed using a layered jelly diffusion method on the MHA medium. The highest antibacterial activity to Staphylococcus aureus was found in whole extract and the 0-20% fraction with the inhibition diameter of 18.63 mm and 13.30 mm, respectively. The toxicity and citotoxicity test against Brine Shrimp Artemia salina Leach and Cervical cancer HeLa cell line indicated the most toxic response was 20-40 % saturation fraction of protein with LC50 about 28.84 g/mL and IC50 26,49 g/mL, respectively. The results indicated that bioactive protein from green algae Turbinaria decurrens is very potential as the basic material for the new anti-bacterial and anticancer drug. |
