Buserelin acetate-induced spermiation in Leptodactylus ocellatus and evaluation of semen characteristics - doi: 10.4025/actascibiolsci.v35i1.14777

Autor: Pereira, Marcelo Maia, Ribeiro Filho, Oswaldo Pinto, Zanuncio, Jose Cola, Troni, Allan Reis, Navarro, Rodrigo Diana, Takamura, Angela Emi
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Zdroj: Acta Scientiarum : Biological Sciences, Vol 35, Iss 1, Pp 1-7 (2012)
Acta Scientiarum. Biological Sciences; Vol 35 No 1 (2013); 1-7
Acta Scientiarum. Biological Sciences; v. 35 n. 1 (2013); 1-7
Acta Scientiarum Biological Sciences
Universidade Estadual de Maringá (UEM)
instacron:UEM
ISSN: 1679-9283
1807-863X
Popis: By understanding the hormonal action behind the induction of spermiation in Leptodactylus ocellatus can help in the collection of semen for artificial insemination and cryopreservation. The experiments were conducted at the Experimental Frog Farm of the Federal University of Viçosa. Seven L. ocellatus males with the following secondary sexual characteristics were selected: black thorns, developed forelimbs, and amplexus reflex. Spermiation was induced in these animals by applying daily 0.4 μg buserelin acetate hormone (GnRHa) for 7 days, being the semen collected 90 min after the application. The volume, color, vigor, motility, sperm concentration, and spermatozoa morphology were subsequently evaluated. Three animals responded to GnRHa induction, enabling the collection of seven samples of semen, and the first sample was collected 97.5h after the first application. The semen of the creole frog presented the following characteristics: an average volume of 0.38 mL, murky color, sperm vigor of 3.71, sperm motility of 77.14%, sperm concentration of 6.60 x 106 SPTZ mL-1, and 69% of normal sperm. GnRHa can induce spermiation in the creole frog. Although the volume of collected semen was low, the color, vigor, motility, concentration, and spermatozoa content showed to be adequate.
Databáze: OpenAIRE
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