Development and Multicentric Validation of a Lateral Flow Immunoassay for Rapid Detection of MCR-1-Producing Enterobacteriaceae

Autor: Volland, Hervé, Dortet, Laurent, Bernabeu, Sandrine, Boutal, Hervé, Haenni, Marisa, Madec, Jean-Yves, Robin, Frédéric, Beyrouthy, Racha, Naas, Thierry, Simon, Stéphanie
Přispěvatelé: Institut de Biologie et de Technologies de Saclay (IBITECS), Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Service de Bactériologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), Commissariat à l'Energie Atomique, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire d'études et de recherches en pathologie bovine et hygiène des viandes, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Télécom ParisTech, CHU Clermont-Ferrand, Résistances émergentes aux antibiotiques, Institut National de la Santé et de la Recherche Médicale (INSERM), OUERTANI, jeannette, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Journal of Clinical Microbiology
Journal of Clinical Microbiology, American Society for Microbiology, 2019, 57 (5), pp.1-8. ⟨10.1128/JCM.01454-18⟩
Journal of Clinical Microbiology, 2019, 57 (5), pp.1-8. ⟨10.1128/JCM.01454-18⟩
ISSN: 0095-1137
DOI: 10.1128/JCM.01454-18⟩
Popis: International audience; Colistin has become a last-resort antibiotic for the treatment of infections caused by highly drug-resistant Gram-negative bacteria. Moreover, it has been widely used in the livestock sector. As a consequence, colistin resistance is emerging worldwide. Among the colistin resistance mechanisms, the spread of the plasmid-encoded colistin resistance gene mcr-1 (mostly in Escherichia coli) is of particular concern due to its increased transferability compared to that of chromosome-encoded resistance. The early detection of MCR-1-producing bacteria is essential to prevent further spread and provide appropriate antimicrobial therapy. Lateral flow immunoassays (LFIAs) were manufactured with selected monoclonal antibodies. A collection of 177 human and 121 animal enterobacterial isolates was tested in a multicentric study. One bacterial colony grown on agar plates was suspended in extraction buffer and dispensed on the cassette. Migration was allowed for 15 min, and the results were monitored by the appearance of a specific band. The positive results showed a pink line resulting in an unambiguous interpretation. All MCR-1-producing isolates were found to be positive by the LFIA, and no false-negative results were observed. Three out of four MCR-2-producing isolates were also found to be positive. Our test does not detect MCR-3-, MCR-4-, or MCR-5-producing isolates. LFIA allows the detection of MCR-1 with 100% sensitivity and 98% specificity. This test is fast, sensitive, specific, easy to use, and cost-effective and can therefore be implemented in any microbiology laboratory worldwide. LFIA is a major tool for the rapid detection and monitoring of MCR-1 producers in humans and animals.
Databáze: OpenAIRE
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