Transcriptome profiling of feeding-to-fasting transition in chicken liver using a chicken 20K oligo microarray
| Autor: | Désert, Colette, Blavy, P., Moreews, F., Duclos, M.J., Le Roy, P., Douaire, M., Diot, C., Lagarrigue, Sandrine |
|---|---|
| Přispěvatelé: | Génétique Animale (GARen), IFR140-Ecole Nationale Supérieure Agronomique de Rennes-AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Ecole Nationale Supérieure Agronomique de Rennes, Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Plant & Animal Genomes, XVI Conference Plant & Animal Genomes, XVI Conference, Jan 2008, San Diego (US), United States Plant & Animal Genomes, XVI Conference, Jan 2008, Inconnu, 2008 |
| Popis: | Résumé : Starvation triggers a complex array of adaptative metabolic responses including energy-metabolic responses, a process which must imply tissue specific alterations in gene expression profiles. In the chicken, liver is a major organ controlling energy metabolism. The present study aimed to describe the evolution of global gene expression profiles in chicken liver during a 48h fasting period. Liver RNA samples were collected from 4 weeks old broilers, fed ad libitum or fasted for 16h or 48h. Following reverse-transcription and Cy dye labelling, the samples were hybridized on chicken 20K oligochips (ARK-genomics) against a reference sample. The data were then normalized by "Lowess-fitness" and analyzed by analysis of variance using LIMMA package. The number of genes altered by fasting increased from 190 at 16h to 611 at 48h (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|