Ratiometric imaging of pH probes
| Autor: | Grillo-Hill, BK, Webb, BA, Barber, DL |
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| Rok vydání: | 2014 |
| Předmět: |
Microscopy
Whole-mount tissue imaging Green Fluorescent Proteins pH measurement Quantitative imaging Biosensing Techniques Genetically encoded pH biosensor Hydrogen-Ion Concentration Reference Standards Fluoresceins Fluorescence Cell Line Drosophila melanogaster In situ imaging Live-cell imaging Animals Ratiometric imaging Biochemistry and Cell Biology Single-Cell Analysis pH-sensitive dyes Fluorescent Dyes Developmental Biology |
| Zdroj: | Grillo-Hill, BK; Webb, BA; & Barber, DL. (2014). Ratiometric Imaging of pH Probes. Methods in Cell Biology, 123, 429-448. doi: 10.1016/B978-0-12-420138-5.00023-9. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/7v5171qm |
| Popis: | Measurement of intracellular pH can be readily accomplished using tools and methods described in this chapter. We present a discussion of technical considerations of various ratiometric pH-sensitive probes including dyes and genetically encoded sensors. These probes can be used to measure pH across physical scales from macroscopic whole-mount tissues down to organelles and subcellular domains. We describe protocols for loading pH-sensitive probes into single cells or tissues and discuss ratiometric image acquisition and analysis. © 2014 Elsevier Inc. |
| Databáze: | OpenAIRE |
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