| Autor: |
Dietrich, Isabelle, Jansen, Stephanie, Fall, Gamou, Lorenzen, Stephan, Rudolf, Martin, Huber, Katrin, Heitmann, Anna, Schicht, Sabine, Ndiaye, El Hadji, Watson, Mick, Castelli, Ilaria, Brennan, Benjamin, Elliott, Richard M., Diallo, Mawlouth, Sall, Amadou A., Failloux, Anna-Bella, Schnettler, Esther, Kohl, Alain, Becker, Stefanie C., Duprex, W. Paul |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
mSphere, Vol 2, Iss 3, p e00090-17 (2017) |
| ISSN: |
2379-5042 |
| Popis: |
The emerging bunyavirus Rift Valley fever virus (RVFV) is transmitted to humans and livestock by a large number of mosquito species. RNA interference (RNAi) has been characterized as an important innate immune defense mechanism used by mosquitoes to limit replication of positive-sense RNA flaviviruses and togaviruses; however, little is known about its role against negative-strand RNA viruses such as RVFV. We show that virus-specific small RNAs are produced in infected mosquito cells, in Drosophila melanogaster cells, and, most importantly, also in RVFV vector mosquitoes. By addressing the production of small RNAs in adult Aedes sp. and Culex quinquefasciatus mosquitoes, we showed the presence of virus-derived Piwi-interacting RNAs (piRNAs) not only in Aedes sp. but also in C. quinquefasciatus mosquitoes, indicating that antiviral RNA interference in C. quinquefasciatus mosquitoes is similar to the described activities of RNAi in Aedes sp. mosquitoes. We also show that these have antiviral activity, since silencing of RNAi pathway effectors enhances viral replication. Moreover, our data suggest that RVFV does not encode a suppressor of RNAi. These findings point toward a significant role of RNAi in the control of RVFV in mosquitoes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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