Proximity labeling: an emerging tool for probing in planta molecular interactions

Autor: Yang, Xinxin, Wen, Zhiyan, Zhang, Dingliang, Li, Zhen, Li, Dawei, Nagalakshmi, Ugrappa, Dinesh-Kumar, Savithramma P, Zhang, Yongliang
Rok vydání: 2021
Předmět:
Zdroj: Plant communications, vol 2, iss 2
Popis: Protein-protein interaction(PPI) networks are key to nearly all aspects of cellular activity. Therefore, the identification of PPIs is important for understanding a specific biological process in an organism. Compared with conventional methods for probing PPIs, the recently described proximity labeling (PL) approach combined with mass spectrometry (MS)-based quantitative proteomics has emerged as a powerful approach for characterizing PPIs. However, the application of PL in planta remains in its infancy. Here, we summarize recent progress in PL and its potential utilization in plant biology. We specifically summarize advances in PL, including the development and comparison of different PL enzymesand the application of PL for deciphering various molecular interactions in different organisms with an emphasis on plant systems.
Databáze: OpenAIRE