Who’s who : Resolving the taxonomy of the causal agent of bacterial leaf spot of lettuce through a polyphasic approach leads to combine Xanthomonas hortorum Vauterin et al. 1995 and Xanthomonas cynarae (Trébaol 2000) Timilsina et al. 2018

Autor: Morinière, Lucas, Burlet, Alexandre, Rosenthal, Emma, Nesme, Xavier, Portier, Perrine, Bull, Carolee, Lavire, Céline, Fischer-Le Saux, Marion, Bertolla, Franck
Přispěvatelé: Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Station expérimentale Rhône-Alpes et Information Légumière, SERAIL, Department of Plant pathology and Environmental Microbiology, Pennsylvania State University (Penn State), Penn State System-Penn State System, Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: EuroXanth COST Action 3rd Annual Conference
EuroXanth COST Action 3rd Annual Conference, Sep 2019, Lednice, Czech Republic
Popis: International audience; Assessment of the taxonomy and diversity of the Xanthomonas strains causing bacterial leaf spot of lettuce (BLSL), commonly referred as Xanthomonas campestris pathovar vitians, has been a long-lasting issue which held back the global efforts made to understand this disease and struggle against the damages it causes in the field. In order to restore sane bases essential to the study of this pathogen, we conducted an extensive polyphasic approach on a panel of strains obtained through sampling campaigns or collection strains acquisitions. Results of a multilocus sequence analysis crossed with pathogenicity assays revealed that BLSL-causing strains formed a monophyletic pathovar sparsely diversified in three clonal groups. However, genome-based phylogenies yielded only two major groups of strains. From the bigger picture perspective, phylogenies exhibited the intermediate position of pathovar vitians between close species X. hortorum and X. cynarae, resulting in a phylogenetic continuum regarding the known diversity of the genus. Finally, whole-genome comparisons of all type, pathotype or representative strains of these two species by multiple overall genome relatedness indices (OGRIs) calculation endorsed the hypothesis of one single specie. Carbon and nitrogen sources utilization tested using Biolog microplates did not revealed any particular phenotypic differences which would have legitimate the distinction into two species. Therefore, we propose the combination of X. hortorum, X. cynarae and X. campestris pv. vitians into a new X. hortorum comb. nov. with CFBP 4925 T = LMG 937 T remaining the type strain, and LMG 938 neoPT = NCPB 2248 neoPT becoming the pathotype strain of X. hortorum pv. vitians.
Databáze: OpenAIRE
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