Physiological and phylogenetic characterization of a stable benzene-degrading, chlorate-reducing microbial community

Autor: Weelink, S.A.B., Tan, N.C.G., Broeke, H. ten, Doesburg, W. van, Langenhoff, A.A., Gerritse, J., Stams, A.J.M.
Přispěvatelé: TNO Bouw en Ondergrond
Jazyk: angličtina
Rok vydání: 2007
Předmět:
DNA
Bacterial
Electrophoresis
molecular cloning
Molecular Sequence Data
bacterium culture
bacterial growth
Environment
biodegradation
Polymerase Chain Reaction
Stenotrophomonas acidaminiphila
benzene
RNA
Ribosomal
16S
Alicycliphilus denitrificans
oxic conditions
Bacteria (microorganisms)
molecular phylogeny
Ecosystem
inorganic salt
Phylogeny
anoxic conditions
Bacteria
Mesorhizobium sp. WG
Benzene degradation
Mesorhizobium
article
anoxia
nucleotide sequence
sequence homology
Sequence Analysis
DNA
denaturing gradient gel electrophoresis
zoogloea resiniphila
bacterial strain
bacterium
phylogenetics
degradation kinetics
Enrichment culture
priority journal
Ecological Microbiology
physiology
Chlorates
microflora
chlorate
microbial community
bacterial gene
Chlorate reduction
Oxidation-Reduction
molecular stability
Zdroj: FEMS Microbiology Ecology, 2, 60, 312-321
FEMS Microbiology Ecology, 60, 2, pp. 312-321
FEMS Microbiology Ecology, 60, 312-321
ISSN: 0168-6496
Popis: A stable anoxic enrichment culture was obtained that degraded benzene with chlorate as an electron acceptor. The benzene degradation rate was 1.65 mM benzene per day, which is similar to reported aerobic benzene degradation rates but 20-1650 times higher than reported for anaerobic benzene degradation. Denaturing gradient gel electrophoresis of part of the 16S rRNA gene, cloning and sequencing showed that the culture had a stable composition after the seventh transfer. Five bacterial clones were further analyzed. Two clones corresponded to bacteria closely related to Alicycliphilus denitrificans K601. The three other clones corresponded to bacteria closely related to Zoogloea resiniphila PIV-3A2w, Mesorhizobium sp. WG and Stenotrophomonas acidaminiphila. DGGE analysis of cultures grown with different electron donors and acceptors indicated that the bacterium related to Alicycliphilus denitrificans K601 is able to degrade benzene coupled to chlorate reduction. The role of the other bacteria could not be conclusively determined. The bacterium related to Mesorhizobium sp. WG can be enriched with benzene and oxygen, but not with acetate and chlorate, while the bacterium related to Stenotrophomonas acidaminophila grows with acetate and chlorate, but not with benzene and oxygen. As oxygen is produced during chlorate reduction, an aerobic pathway of benzene degradation is most likely. © 2007 Federation of European Microbiological Societies.
Databáze: OpenAIRE
Externí odkaz: