Dexketoprofen trometamol-loaded poly-lactic-co-glycolic acid (PLGA) nanoparticles: Preparation, in vitro characterization and cyctotoxity

Autor: Öztürk, A. Alper, Martín Banderas, Lucía, Cayero Otero, María Dolores, Yenilmez, Evrim, Şenel, Behiye, Yazan, Yasemin
Přispěvatelé: Universidad de Sevilla. Departamento de Farmacia y Tecnología Farmacéutica, Anadolu Üniversitesi, Eczacılık Fakültesi, Farmasötik Teknoloji Anabilim Dalı, Yazan, Yasemin
Rok vydání: 2019
Předmět:
Zdroj: idUS: Depósito de Investigación de la Universidad de Sevilla
Universidad de Sevilla (US)
Tropical Journal of Pharmaceutical Research; Vol 18, No 1 (2019); 1-11
idUS. Depósito de Investigación de la Universidad de Sevilla
instname
ISSN: 1596-9827
1596-5996
Popis: WOS: 000457490100001
Purpose: To design, formulate and characterize sustained-release formulations of dexketoprofen trometamol (DT) nanoparticles (NPs) Methods: Dexketoprofen trometamol (DT)-loaded poly(lactic-co-glycolic acid) (PLGA) NPs were produced by double emulsion-solvent evaporation method. The NPs were variously characterized for drug loading and release, particle profile, as well as by thermal analysis, x-ray difraction (XRD), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance analysis (H-1-NMR). Furthermore, the NPs were evaluated for cytotoxicity against NIH-3T3 cells by 3-(4, 5-dimethylthiazol-2-Yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Results: The DT-loaded NPs demonstrated nanostructural characteristics and extended drug release. Particle size was in the range of 243 and 295 nm which remained unchanged in drug stability testing in simulated gastrointestinal media. Encapsulation efficiency ranged from 49 - 64 % for all the formulations. Higuchi and Korsmeyer-Peppas were the best-fit release kinetic models for the NPs containing 5 and 10 % DT, respectively. The NPs with 10 % DT presented no significant cytotoxicty at the doses and periods studied. Conclusion: Stable and non-toxic DT NPs with potential for sustained and controlled release of the drug have been successfully developed.
Anadolu University Scientific Research Project Foundation [1708S471]; Faculty of Science
This study was financed by Anadolu University Scientific Research Project Foundation (no. 1708S471). The authors would like to thank Abdi Ibrahim (Istanbul, Turkey) for kindly providing DT. Faculty of Science is acknowledged for XRD, DOPNALAB Faculty of Pharmacy for FTIR and 1H-NMR and AUBIBAM for SEM analyses. We also thank University of Seville, Faculty of Pharmacy, Department of Pharmacy and Pharmaceutical Technology for making available their facilities for this work.
Databáze: OpenAIRE
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