Isolation of Saprolegnia aenigmatica oomycetes and protocol for experimental infection of pacu (Piaractus mesopotamicus)

Autor: Carraschi, Silvia Patrícia, Garlich, Nathalia [UNESP], de Souza-Pollo, Andressa [UNESP], Pereira, Daniela Isabel Brayer, da Cruz, Claudinei, Ranzani-Paiva, Maria José Tavares
Přispěvatelé: Parque da Água Branca, Universidade de Araraquara, Universidade Estadual Paulista (UNESP), Universidade Federal de Pelotas, Centro Universitário da Fundação Educacional de Barretos
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Acta Scientiarum. Biological Sciences; Vol 40 (2018): Publicação Contínua; e38186
Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e38186
Acta Scientiarum Biological Sciences
Universidade Estadual de Maringá (UEM)
instacron:UEM
Acta Scientiarum : Biological Sciences, Vol 40, Iss 1 (2018)
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1807-863X
1679-9283
Popis: Made available in DSpace on 2022-04-28T19:26:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Successful disease treatment depends on molecular studies under indoor conditions with experimental infection protocols that facilitate understanding the disease and the drug`s efficacy. The internal transcribed spacer (ITS) region was sequenced from three isolates, which were identified as Saprolegnia aenigmatica. Subsequently, healthy fish were immunosuppressed with dexamethasone (1.2 mg kg-1) and descaled to the skin using a sharp scalpel. These individuals were isolated in individual aquariums maintained at 22°C. Individuals in one group were subcutaneously inoculated with 9,000 zoospores (DDZ treatment), a second group was exposed to oomycetes in water with three colonized baits (DDB), a third group was maintained in water without zoospores (DD), and a control group (C) consisted of healthy animals. After 48 and 96 hours, two animals from each group were euthanized for fungal re-isolation. The fish from groups DD and C did not show clinical signs, and no oomycetes were isolated. The animals from the DDZ and DDB groups showed cotton-wool-like masses on the skin, and S. aenigmatica was re-isolated. Thus, for infection using zoospores or baits parasitized by S. aenigmatica, an immunosuppressor (dexamethasone) and a sharp scalpel can be used effectively to establish an experimental infection in P. mesopotamicus. Instituto de Pesca Parque da Água Branca, Avenida Francisco Matarazzo, 455 Universidade de Araraquara, Avenida Maria Antonia Camargo de Oliveira, 170 Núcleo de Estudos em Pesquisa Ambiental em Matologia Faculdade de Ciências Agronômicas e Veterinárias Universidade Estadual Paulista Laboratório de Epidemiologia Molecular Universidade Estadual Paulista Universidade Federal de Pelotas Centro Universitário da Fundação Educacional de Barretos Núcleo de Estudos em Pesquisa Ambiental em Matologia Faculdade de Ciências Agronômicas e Veterinárias Universidade Estadual Paulista Laboratório de Epidemiologia Molecular Universidade Estadual Paulista FAPESP: 2013/25113-2 FAPESP: 2015/10645-4
Databáze: OpenAIRE
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