UHPLC-HRMS determination of palytoxin and ovatoxins from Ostreopsis cf. ovata, a marine microalgae blooming in a Northwestern Mediterranean beach
| Autor: | Moyano, Encarnación, Alechaga, Élida, Caixach Gamisans, Josep, Lacorte Bruguera, Silvia, Palacios, Óscar, Farràs, Jaume, Vila, Magda, Viure Feliu, Laia, Berdalet, Elisa |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España) |
| Rok vydání: | 2016 |
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| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 21st International Mass Spectrometry Conference (IMSC 2016), 20-27 August 2016, Toronto, Canada Introduction: Blooms of Ostreopsis cf. ovata (palytoxin/ovatoxins-producing microalgae) are spreading to coastal areas of temperate latitudes and constitute a health and environmental emergent problem. The risks posed by these events will only be determined if toxin patterns are identified and quantified. Nevertheless, the mass spectrometry characterization of palytoxin/ovatoxins is already a challenge. Methods: Ultra-high performance reversed phase liquid chromatography coupled to electrospray-high resolution mass spectrometry (UHPLC- HRMS) was applied for the reliable identification and determination of palytoxin/ovatoxins. Extraction, UHPLC and HRMS protocols were optimized to quantify the different toxins along an Ostreopsis cf. ovata bloom in Sant Andreu de Llavaneres beach (Barcelona, Spain). Results: Ovatoxins are complex macromolecules closely related to Palytoxin, whose mass spectrometry characterization is already a challenge. The complex electrospray mass spectra show multiply charged ions, mixed cationized species and adducts, thus requiring efficient chromatographic separations of potential interfering species and the careful selection of diagnostic ions for their analysis. The relative intensity variation of cationized species from sample-to-sample and from standards hinders quantitative analysis, thus entailing the use of integrated signals from selected non-interfering ions. Microalgae samples from a Mediterranean beach were analyzed during an Ostreopsis bloom in 2015. Putative palytoxin and ovatoxins were detected, being ovatoxin-a the dominant component. The highest concentration per cell almost coincided with maximum Ostreopsis abundances. Novel Aspect: The improved UHPLC-HRMS method evidences the presence of putative palytoxin and ovatoxins in the Ostreopsis bloom in a NW Mediterranean beach. This work was financed by the Spanish Ministry of Economy and Competitiveness (MINECO) under the projects CTQ2015-63968-C2-1-P and OSTREORISK CTM2014-53818-R |
| Databáze: | OpenAIRE |
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