Oxidation of model lipids representative for main paper pulp lipophilic extractives by the laccase-mediator system
| Autor: | Molina, Setefilla, Rencoret, Jorge, Río Andrade, José Carlos del, Lomascolo, Anne, Record, Eric, Martínez, Ángel T., Gutiérrez Suárez, Ana |
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| Rok vydání: | 2008 |
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| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Several model lipids representative for main paper pulp lipophilic extractives - including alkanes, fatty alcohols, fatty acids, resin acids, free and esterified sterols, and triglycerides – were treated with Pycnoporus cinnabarinus laccase in the presence of 1-hydroxybenzotriazole as mediator. The reaction products were analyzed by GC and GC-MS. The laccase alone decreased the amount of some unsaturated lipids, however, the most rapid and extensive lipid modification was obtained with the laccase-mediator system. Most unsaturated lipids were largely oxidized and the dominant oxidation products detected were epoxy and hydroxy-fatty acids from fatty acids, and free and esterified 7-ketosterols and steroid ketones from sterols and sterol esters. In contrast, saturated lipids were not modified, although some of them were oxidized when the enzymatic reactions were carried out in the presence of unsaturated lipids. The results obtained are discussed in the context of enzymatic control of pitch deposits, to explain the removal of lipid mixtures during laccase-mediator treatment of different pulp types. This study was funded by the BIORENEW EU-project (NMP2-CT-2006-026456) and the Spanish MEC (BIO2007-28719-E). Beldem (Andenne, Belgium) is acknowledged for laccase supply. S.M. thanks the Spanish CSIC and CELESA for an I3P contract and J.R thanks the CSIC for an I3P fellowship. |
| Databáze: | OpenAIRE |
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