Exploring collagen parameters in pure special types of invasive breast cancer
| Autor: | Natal, Rodrigo de Andrade, 1991, Silva, Geisilene Russano de Paiva, 1977, Marenco Camacho, Ludwing Ferney, 1990, Alvarenga, César Augusto, 1975, Vargas, Renato Flora, 1962, Derchain, Sophie Françoise Mauricette, 1959, Sarian, Luís Otávio Zanatta, 1974, César, Carlos Lenz, 1955, Vassallo, José, 1957 |
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| Přispěvatelé: | UNIVERSIDADE ESTADUAL DE CAMPINAS |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Repositório da Produção Científica e Intelectual da Unicamp Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP Repositório Institucional da Unicamp |
| Popis: | Agradecimentos: The authors thank the National Institute of Science and Technology on Photonics Applied to Cell Biology (INFABiC) at the State University of Campinas for access to equipment and assistance; INFABiC is co-funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (08/57906-3) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (573913/2008-0). C. L. Cesar also acknowledges the grant by FAPESP (11/51959-0) and CNPq (312049/2014-5) Abstract: One of the promising tools to evaluate collagen in the extracellular matrix is the second-harmonic generation microscopy (SHG). This approach may shed light on the biological behavior of cancers and their taxonomy, but has not yet been applied to characterize collagen fibers in cases diagnosed as invasive breast carcinoma (BC) of histological special types (IBC-ST). Tissue sections from 99 patients with IBC-ST and 21 of invasive breast carcinoma of no special type (IBC-NST) were submitted to evaluation of collagen parameters by SHG. Tissue microarray was performed to evaluate immunohistochemical-based molecular subtype. In intratumoral areas, fSHG and bSHG (forward-SHG and backward-SHG) collagen parameters achieved their lowest values in mucinous, papillary and medullary carcinomas, whereas the highest values were found in classic invasive lobular and tubular carcinomas. Unsupervised hierarchical cluster analysis and minimal spanning tree using intratumoral collagen parameters allowed the identification of three main groups of breast cancer: group A (classic invasive lobular and tubular carcinomas); group B (IBC-NST, metaplastic, invasive apocrine and micropapillary carcinomas); and group C (medullary, mucinous and papillary carcinomas). Our findings provide further characterization of the tumor microenvironment of IBC-ST. This understanding may add information to build more consistent tumor categorization and to refine prognostication CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP Aberto |
| Databáze: | OpenAIRE |
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