| Autor: |
Nathan E. Barlow, Guido Bolognesi, Stuart Haylock, Anthony J. Flemming, Nicholas J. Brooks, Laura M. C. Barter, Oscar Ces |
| Přispěvatelé: |
Engineering & Physical Science Research Council (EPSRC), Engineering & Physical Science Research Council (E, Commission of the European Communities, Engineering and Physical Sciences Research Council, Biotechnology and Biological Sciences Research Council (BBSRC) |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
| Popis: |
A new rheological droplet interface bilayer (rheo-DIB) device is presented as a tool to apply shear stress on biological lipid membranes. Despite their exciting potential for affecting high-throughput membrane translocation studies, permeability assays conducted using DIBs have neglected the effect of the unstirred water layer (UWL). However as demonstrated in this study, neglecting this phenomenon can cause significant underestimates in membrane permeability measurements which in turn limits their ability to predict key processes such as drug translocation rates across lipid membranes. With the use of the rheo-DIB chip, the effective bilayer permeability can be modulated by applying shear stress to the droplet interfaces, inducing flow parallel to the DIB membranes. By analysing the relation between the effective membrane permeability and the applied stress, both the intrinsic membrane permeability and UWL thickness can be determined for the first time using this model membrane approach, thereby unlocking the potential of DIBs for undertaking diffusion assays. The results are also validated with numerical simulations. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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