SARS-CoV-2 infection of human iPSC-derived cardiac cells reflects cytopathic features in hearts of patients with COVID-19
| Autor: | Perez-Bermejo, Juan A, Kang, Serah, Rockwood, Sarah J, Simoneau, Camille R, Joy, David A, Silva, Ana C, Ramadoss, Gokul N, Flanigan, Will R, Fozouni, Parinaz, Li, Huihui, Chen, Pei-Yi, Nakamura, Ken, Whitman, Jeffrey D, Hanson, Paul J, McManus, Bruce M, Ott, Melanie, Conklin, Bruce R, McDevitt, Todd C |
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| Rok vydání: | 2021 |
| Předmět: |
Cells
Induced Pluripotent Stem Cells Cardiovascular Medical and Health Sciences Clinical Research Humans 2.1 Biological and endogenous factors Aetiology Lung Myocytes Cultured Stem Cell Research - Induced Pluripotent Stem Cell - Human Stem Cell Research - Induced Pluripotent Stem Cell SARS-CoV-2 Myocardium COVID-19 Heart Pneumonia Biological Sciences Stem Cell Research Infectious Diseases Heart Disease Emerging Infectious Diseases Autopsy Transcriptome Cardiac |
| Zdroj: | Science translational medicine, vol 13, iss 590 |
| Popis: | Although coronavirus disease 2019 (COVID-19) causes cardiac dysfunction in up to 25% of patients, its pathogenesis remains unclear. Exposure of human induced pluripotent stem cell (iPSC)-derived heart cells to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) revealed productive infection and robust transcriptomic and morphological signatures of damage, particularly in cardiomyocytes. Transcriptomic disruption of structural genes corroborates adverse morphologic features, which included a distinct pattern of myofibrillar fragmentation and nuclear disruption. Human autopsy specimens from patients with COVID-19 reflected similar alterations, particularly sarcomeric fragmentation. These notable cytopathic features in cardiomyocytes provide insights into SARS-CoV-2-induced cardiac damage, offer a platform for discovery of potential therapeutics, and raise concerns about the long-term consequences of COVID-19 in asymptomatic and severe cases. |
| Databáze: | OpenAIRE |
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