Ulvane, un polysaccharide sulfaté d’Ulva armoricana, stimule la réponse immunitaire intestinale par un mécanisme impliquant la voie de signalisation TLR4/Akt
| Autor: | Berri, Mustapha, Olivier, Michel, Holbert, Sébastien, Dupont, Joëlle, Demais, Hervé, Le Goff, Matthieu, Nyvall Collen, Pi |
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| Přispěvatelé: | Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Biovet Conseil, 'Pôle biotechnologique' du Haut du Bois, Amadéite SAS, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut du Porc (IFIP). FRA. Institut National de la Recherche Agronomique (INRA), FRA., ProdInra, Archive Ouverte, Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | francouzština |
| Rok vydání: | 2017 |
| Předmět: |
filtration tangentielle
immunopotentiation [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health [SDV.IMM] Life Sciences [q-bio]/Immunology ulvane réponse immunitaire RT-qPCR [SDV.BA.MVSA] Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health indirect elisa immunologic reactions green algae crossflow filtration voie de signalisation [SDV.IMM]Life Sciences [q-bio]/Immunology immunostimulation algue verte polysaccharide sulfate technique elisa |
| Zdroj: | 49. Journées de la Recherche Porcine 49. Journées de la Recherche Porcine, Jan 2017, Paris, France. IFIP-Institut du Porc, 49, 2017, Journées de la Recherche Porcine en France 49. Journées de la Recherche Porcine, Jan 2017, Paris, France. IFIP-Institut du Porc, Journées de la Recherche Porcine en France, 49, 2017, Journées de la Recherche Porcine en France |
| Popis: | Session : Santé animale; The biological activities of water-soluble sulfated polysaccharides of green algae (Ulvans) are explored with the aim of their being used as bioactive molecules for human and animal health. A purified fraction of ulvans was prepared from the green algae Ulva armoricana harvested in the Brittany region (France) and tested for its capacity to stimulate the immune response of the gut using an in vitro system of porcine intestinal epithelial cells (IPEC-1). RT-qPCR and ELISA analysis showed a significant increase in mRNA and protein expression of cytokines such as CCL20, IL8, and TNFalpha. By using human embryonic kidney HEK293 reporter cell lines for pattern recognition receptors, ulvan was found to stimulate mostly TLR4. We also examined this fraction’s effect on different signaling pathways involved in activating cytokine gene expression. Western blot analysis of ulvan-treated HEK293-TLR4 cells showed an increase in Akt and the p65 subunit of the nuclear factor-κB (NFκB) phosphorylation. Inhibition of Akt phosphorylation with specific inhibitor abrogated ulvan enhancement of IL-8 secretion. The overall results showed that ulvan is an immunostimulator compound by itself, and furthermore, it could be used to encapsulate and deliver TLR ligands to relevant immune cells to strongly enhance adjuvanticity and immunity in vaccination strategies. |
| Databáze: | OpenAIRE |
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