The effect of estrogen receptor genotypes on the number of stillborn and mummified piglets in Topigs 20 sows

Autor: Sven Menčik, Vlado Vuković, Mario Modrić, Marija Špehar, Mario Ostović, Velimir Sušić, Igor Štoković, Krešimir Salajpal, Hrvoje Kabalin, Anamaria Ekert Kabalin
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Veterinarski arhiv
Volume 86
Issue 3
ISSN: 0372-5480
1331-8055
Popis: Steroidni hormoni imaju ključnu ulogu u postnatalnom razvitku i fiziologiji rasplođivanja ženskih jedinki. Djelovanje ovih hormona očituje se kroz podražaj njihovog receptora. Odsječak gena za estrogeni receptor među prvim je i najistraživanijim kandidatnim genima za reprodukcijsku učinkovitost svinja, naročito za pokazatelje veličine legla. Cilj ovoga istraživanja bio je utvrditi polimorfizam gena estrogenog receptora uporabom restrikcijske endonukleaze i njegovu povezanost s brojem mrtvooprasenih (BMO) i mumificiranih (BMUM) odojaka u prvo-, drugo-, treće- i višepraskinja te skupnom analizom prasenja. Istraživanjem je bila obuhvaćena 101 krmača hibrida Topigs 20 od prvog to sedmog prasenja. Polimorfizam gena estrogenog receptora utvrđen je cijepanjem umnoženih odsječaka gena restrikcijskim enzimom PvuII nakon postupka lančane reakcije polimerazom. Utvrđena je prisutnost dvaju alela, A i B, te triju genotipova. Učestalost genotipova i alela provjerena je χ2-testom. Usporedbom je utvrđeno značajno odstupanje od Hardy-Weinbergova zakona utvrđenih i očekivanih frekvencija genotipova. Prikupljeni podatci analizirani su pomoću općeg linearnog modela izračuna (GLM). Statističkom obradom podataka utvrđena je značajna razlika (P
Steroid hormones such as estrogen play a central role in the postnatal female physiology and their effects are exerted through its receptors. The estrogen receptor gene is one of the first and most extensively investigated candidate genes for reproductive traits in pigs, especially for litter size. The aim of the study was to investigate the estrogen receptor gene polymorphism using endonuclease PvuII, and its association with the number of stillborn (NSB) and the number of mummified (NMUM) piglets in the first, second, third and subsequent parities and in overall parities. Topigs 20 sows (n = 101) from 1st to 7th parities were analyzed. Estrogen receptor genotypes were detected by the polymerase chain reaction-restriction fragment length polymorphism method. Two alleles (A and B) were identified with three genotypes. Alleles (A and B) and genotype frequencies were determined. Comparison of the observed and expected genotype frequencies was performed using the χ2-test and considerable deviation from the Hardy-Weinberg principle was found. All data were analyzed using the General Linear Model. Statistical analysis showed a significant difference (P
Databáze: OpenAIRE
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