In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy
| Autor: | Venturini, James [UNESP], Soares, Cléverson Teixeira, Belone, Andréa de Faria Fernandes, Barreto, Jaison Antonio, Ura, Somei, Lauris, José Roberto Pereira, Vilani-Moreno, Fátima Regina |
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| Přispěvatelé: | Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp), Universidade Estadual Paulista (UNESP), Instituto Lauro de Souza Lima |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Scopus |
| Popis: | Made available in DSpace on 2013-08-12T18:04:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-03-01 Made available in DSpace on 2013-09-30T18:19:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-03-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:34:41Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T13:34:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-03-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Objective: We investigated the in vitro and skin lesions production of cytokines in non-treated borderline tuberculoid (BT) and borderline lepromatous (BL) patients.Patients and Methods: Seven untreated, non-reactional BT patients and 12 untreated, non-reactional BL patients were studied. Levels of the cytokines IFN-gamma, IL-10, TGF-beta 1 and TNF-alpha were measured in supernantant of peripheral blood mononuclear cells (PBMC) cultures, stimulated with specific M. leprae antigen (sonicated and whole). The cytokines iNOS, IL-10 and TGF-beta 1 were detected by immunohistochemistry in skin biopsies.Results: BT patients produced higher levels of IFN-gamma than BL patients; iNOS expression in skin lesions was also higher in BT patients. TGF-beta 1 was detected in more cells in BL patients; IL-10 expression was similar in both groups. There was a negative correlation between iNOS and TGF-beta 1 expression in skin biopsies, positive correlation between TGF-beta 1 in skin lesions and bacillary index, as well as positive correlation between iNOS detected in skin biopsies and PBMC IFN-gamma production.Conclusions: The BT patients had a mainly a Th1-profile of cytokines in their skin lesions and BL patients had aTh2 profile. Inst Lauro de Souza Lima, Equipe Tecn Biol, BR-17034971 Bauru, SP, Brazil UNESP, Fac Med Botucatu, Programa Posgrad Doencas Trop, Botucatu, SP, Brazil Inst Lauro de Souza Lima, Equipe Tecn Patol, BR-17034971 Bauru, SP, Brazil Inst Lauro de Souza Lima, Serv Epidemiol, BR-17034971 Bauru, SP, Brazil Inst Lauro de Souza Lima, Div Pesquisa & Ensino, BR-17034971 Bauru, SP, Brazil Univ São Paulo, Fac Odontol Bauru, Bauru, SP, Brazil UNESP, Fac Med Botucatu, Programa Posgrad Doencas Trop, Botucatu, SP, Brazil |
| Databáze: | OpenAIRE |
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