Ectopic expression in the giant fiber system of Drosophila reveals distinct roles for roundabout (Robo), Robo2, and Robo3 in dendritic guidance and synaptic connectivity
| Autor: | Tanja Godenschwege, Simpson, Jh, Shan, Xl, Bashaw, Gj, Goodman, Cs, Murphey, Rk |
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| Rok vydání: | 2002 |
| Předmět: |
dendrites
1.1 Normal biological development and functioning Amino Acid Motifs Gene Expression Genetically Modified Nerve Tissue Proteins slit Medical and Health Sciences Animals Genetically Modified Nerve Fibers Immunologic roundabout robo synapse Underpinning research Receptors Reaction Time Animals Drosophila Proteins Receptors Immunologic commissureless Motor Neurons axon Neurology & Neurosurgery Psychology and Cognitive Sciences Pupa Neurosciences Membrane Proteins Excitatory Postsynaptic Potentials giant fiber Axons Electric Stimulation Phenotype Synapses Neurological Insect Proteins Drosophila guidance |
| Zdroj: | The Journal of neuroscience : the official journal of the Society for Neuroscience, vol 22, iss 8 Scopus-Elsevier ResearcherID Godenschwege, Tanja A; Simpson, Julie H; Shan, Xiaoliang; Bashaw, Greg J; Goodman, Corey S; & Murphey, Rodney K. (2002). Ectopic expression in the giant fiber system of Drosophila reveals distinct roles for roundabout (Robo), Robo2, and Robo3 in dendritic guidance and synaptic connectivity.. The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(8), 3117-3129. doi: 10.1523/jneurosci.22-08-03117.2002. UC Santa Barbara: Retrieved from: http://www.escholarship.org/uc/item/23p4k5v4 |
| Popis: | The Roundabout (Robo) receptors have been intensively studied for their role in regulating axon guidance in the embryonic nervous system, whereas a role in dendritic guidance has not been explored. In the adult giant fiber system of Drosophila, we have revealed that ectopic Robo expression can regulate the growth and guidance of specific motor neuron dendrites, whereas Robo2 and Robo3 have no effect. We also show that the effect of Robo on dendritic guidance can be suppressed by Commissureless coexpression. Although we confirmed a role for all three Robo receptors in giant fiber axon guidance, the strong axon guidance alterations caused by overexpression of Robo2 or Robo3 have no effect on synaptic connectivity. In contrast, Robo overexpression in the giant fiber seems to directly interfere with synaptic function. We conclude that axon guidance, dendritic guidance, and synaptogenesis are separable processes and that the different Robo family members affect them distinctly. |
| Databáze: | OpenAIRE |
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