Prevalence and Antimicrobial Resistance of Thermophilic Campylobacter Isolates from Raw Chicken Meats
Autor: | Issa, G., Basaran Kahraman, B., Adiguzel, M.C., Yilmaz Eker, F., Akkaya, E., Bayrakal, G.M., Koluman, Ahmet, Kahraman, T. |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
kanamycin
antibiotic resistance Veterinary medicine prevalence Campylobacter coli genetic analysis Raw chicken meat gentamicin minimum inhibitory concentration Antimicrobial resistance Article animal tissue Campylobacter jejuni pcr ciprofloxacin bacterium isolation SF600-1100 antimicrobial resistance gene tetracycline raw chicken meat nonhuman Campylobacter nalidixic acid campylobacter broth dilution mismatch amplification mutation assay antibiotic sensitivity PCR antimicrobial resistance gene erythromycin food contamination bacterium identification chicken meat multiplex polymerase chain reaction |
Zdroj: | Kafkas Universitesi Veteriner Fakultesi Dergisi, Vol 24, Iss 5, Pp 701-707 (2018) |
ISSN: | 1309-2251 1300-6045 |
Popis: | Globally, the spread of antibiotic resistance via chicken meat consumption cause serious public health concerns. With this respect, the current study aimed to investigate the prevalence of thermophilic Campylobacter species isolated from raw meat chicken samples and their genetic determinants of resistance to various classes of antibiotics. A total of 540 chicken raw meat samples collected from various supermarkets and slaughterhouses in Istanbul, Turkey were analyzed according to EN ISO 10272-1:2006 standard procedure. For identification of the genus and species of the isolates, multiplex PCR assay was held. Minimum inhibitory concentrations of the antimicrobial agents (nalidixic acid, ciprofloxacin, tetracycline, gentamicin, kanamycin, and erythromycin) were initially determined using the broth microdilution method. In addition, the genetic determinants of antimicrobial resistance were investigated by PCR assays. In total, 357 (66.1%) Campylobacter isolates were obtained including 268 Campylobacter jejuni and 89 Campylobacter coli. Resistance to quinolones (nalidixic acid and ciprofloxacin) was the most common in all strains (80.1%), followed by resistance to tetracycline’s (70.3%). The lowest resistance was determined as resistance to kanamycin (4.2%). Gentamicin and erythromycin resistance was not observed in this study. Only five C. coli isolate (1.4%) was classified as multidrug resistant. On the basis of these data, execute widely presence of antimicrobial resistance to quinolones and tetracycline’s in C. jejuni and C. coli isolates from chicken raw meat samples and emphasizes that further multidisciplinary studies and novel strategies in the concept of ‘One Health’ are needed. © 2018, Veteriner Fakultesi Dergisi. All rights reserved. |
Databáze: | OpenAIRE |
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