Venous Air Embolism and Complement-driven Thromboinflammation. In vitro human whole blood studies and in vivo porcine studies on the effect of air emboli on the complement system, cytokine network, and the hemostasis
| Autor: | Storm, Benjamin |
|---|---|
| Přispěvatelé: | Mollnes, Tom Eirik |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
DOKTOR-003
VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Medisinsk immunologi: 716 VDP::Medisinske Fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Patofysiologi: 721 VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710::Physiopathology: 721 VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Anestesiologi: 765 VDP::Medical disciplines: 700::Clinical medical disciplines: 750::Anesthesiology: 765 VDP::Medical disciplines: 700::Basic medical dental and veterinary science disciplines: 710::Medical immunology: 716 |
| Popis: | Background: Air embolism may complicate many medical procedures and cause vascular occlusion, organ infarctions, or death. In vitro, air triggers the alternative complement pathway and activates platelets. Previous studies of air emboli were conducted in serum, plasma, or heparin anticoagulated whole blood, with ambient air present in tubes, precluding detailed examination of thromboinflammation. The role of complement in air-induced thromboinflammation has not previously been examined in vivo in minimally anticoagulated large animal model. Thus, this project aimed to examine the effect of avoiding ambient air during in vitro blood incubations and to elucidate the air-induced thromboinflammation both in vitro and in vivo. Methods: In vitro, lepirudin anticoagulated human whole blood from 16 donors was either incubated for 180 minutes with air emboli and inhibitors of complement C3 and C5, C5a receptor 1, and the toll-like receptor co-receptor cluster of differentiation 14, or without air. Blood was analyzed for complement activation products, cytokines, tissue factor, β-thromboglobulin, and prothrombin fragment 1+2. In vivo, air was infused through an ear vein in 29 pigs for 300 minutes. Hemostasis was monitored using rotational thromboelastometry and thrombin-antithrombin complex. Blood and lung tissue were analyzed for complement activation products and cytokines. Results: In vitro and in vivo, air emboli triggered a C3-driven thromboinflammation without correlating terminal pathway activation. In vitro, C3 inhibition, and to a lesser degree, C5 inhibition attenuated cytokine release, and C3 and C5 inhibition equally reduced coagulation, but neither reduced the platelet activation. Avoiding ambient air during in vitro incubations reduced complement activation. In vivo, air embolism resulted in leukocytosis, hemostasis, increased proinflammatory cytokines and complement activation product C3a, but not TCC in the lung tissue. Conclusion: Air embolism triggered a complement C3-driven thromboinflammation in vitro and in vivo. During in vitro blood incubations, avoiding ambient air attenuated and C3 inhibition reduced thromboinflammation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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