A fluorescent infectious clone of Turnip yellows virus to study the effect of aphid infestation on virus distribution in planta
| Autor: | Boissinot, Sylvaine, Pichon, Elodie, Sorin, Céline, Scheidecker, Danièle, Ziegler Graff, Véronique, Brault, Veronique |
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| Přispěvatelé: | Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université de Strasbourg (UNISTRA), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institute of Plant Sciences, Agricultural Research Organization-The Volcani Center, Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), ProdInra, Archive Ouverte |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology
aphide [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology viruses plant louse fungi [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology food and beverages virus de la mosaïque jaune du navet protection des cultures [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology TuYV turnip yellow mosaic virus |
| Zdroj: | 3. Hemipteran-Plant Interactions Symposium. HPIS 2017 3. Hemipteran-Plant Interactions Symposium. HPIS 2017, Jun 2017, Madrid, Spain. 234 p., 2017, HPIS 2017 3rd Hemipteran-Plant Interactions Symposium : book of abstracts 3. Hemipteran-Plant Interactions Symposium. HPIS 2017, Jun 2017, Madrid, Spain. 234 p., 2017 |
| Popis: | BGPI : équipe 2; Turnip yellows virus (TuYV) is a polerovirus (family Luteoviridae) restricted to phloem tissue and obligatorily transmitted by aphids in a circulative and non-propagative mode. This virus infects several plant species of economic importance. The icosahedral viral particles contain a single positive strand RNA genome. In order to track virus movement in plants and to measure the impact of aphids presence on virus distribution in plants, a GFP-tagged virus was engineered (TuYV-GFP). In this construct, the GFP sequence was inserted in a non-structural protein sequence involved, to some extent, in virus movement. The fluorescent signal is expected to label virus replicating cells. The TuYV-GFP sequence was introduced into a binary vector to inoculate several plant species by agro infiltration. TuYV-GFP was able to replicate and to move in A. thaliana, N.benthamiana and M. perfoliata. Accumulation of TuYV-GFP was however reduced when compared to the wild type virus. The impaired ability of the recombinant virus to reach efficiently non inoculated leaves is likely due to the insertion of the GFP sequence in the viral genome. The TuYVGFP genome was stable in N. benthamiana while it was subjected to partial deletions in A. thalianaand in M. perfoliata. Nevertheless, we observed fluorescent phloem cells in non-inoculated leaves of the three plant species. Importantly, fluorescent phloem cells were also observed after virus acquisition and inoculation by aphids, both in inoculated and non-inoculated leaves. This major breakthrough in the study of poleroviruses persistently transmitted by aphids will now be used to address whether the virus localisation in planta is affected following aphid infestation. |
| Databáze: | OpenAIRE |
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