| Autor: |
Cornaglia, Matteo, Krishnamani, Gopalan Hariharan, Meurville, Marie-Pierre, Mouchiroud, Laurent, Lehnert, Thomas, Auwerx, Johan, Gijs, Martinus A. M. |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Proceed. The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), San Antonio, TX, USA. |
| Popis: |
We describe a microfluidic platform for the automated culture, treatment and long-term highresolution imaging of Caenorhabditis elegans nematodes under normal physiological conditions. Our device features: (i) a microfluidic design tailored for the isolation of L4 larvae from a mixed larval population and for successive culture and treatment; (ii) a worm immobilization method, based on the thermo-reversible gelation of a biocompatible polymer inside the microfluidic chip, thereby enabling high-resolution imaging; (iii) an integrated temperature control system, both to ensure viable environmental conditions for C. elegans culture and to steer the worm immobilization/release process. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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