Efficient production of Arthromyces ramosus peroxidase by Aspergillus awamori
| Autor: | Lokman, B.C., Joosten, V., Hovenkamp, J., Gouka, R.J., Verrips, C.T., Hondel, C.A.M.J.J. van den |
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| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
culture medium
Heme limitation xylan endo 1 3 beta xylosidase peroxidase Heme Molecular weight Western blotting arthromyces ramosus Growth kinetics enzyme analysis Heterologous protein production nonhuman Enzyme kinetics fungus Fungi article 'Arthromyces ramosus' Blotting Northern fungal enzyme Blotting Southern Aspergillus priority journal Western blot analysis Aspergillus awamori enzyme synthesis Fermentation Cell culture Mitosporic Fungi Promoter Regions (Genetics) Biotechnology Plasmids |
| Zdroj: | Journal of Biotechnology, 2, 103, 183-190 |
| Popis: | The heterologous production of Arthromyces ramosus peroxidase (ARP) was analysed in the filamentous fungus Aspergillus awamori under control of the inducible endoxylanase promoter. Secretion of active ARP was achieved up to 800 mg l-1 in shake flask cultures. Western blot analysis showed that an rARP product of the correct molecular weight was produced. In contrast to several other studies about heterologous production of heme containing peroxidases, our results suggest that in A. awamori no heme limitation exists during overproduction of ARP. © 2003 Elsevier Science B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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