Human induced pluripotent stem cell-based microphysiological tissue models of myocardium and liver for drug development
| Autor: | Mathur, A, Loskill, P, Hong, S, Lee, J, Marcus, SG, Dumont, L, Conklin, BR, Willenbring, H, Lee, LP, Healy, KE |
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| Rok vydání: | 2013 |
| Předmět: |
Technology
Induced Pluripotent Stem Cells Bioengineering Regenerative Medicine Medical and Health Sciences Myoblasts Rare Diseases Humans Hypoglycemic Agents Stem Cell Research - Embryonic - Human Cyclooxygenase 2 Inhibitors Stem Cell Research - Induced Pluripotent Stem Cell - Human Stem Cell Research - Induced Pluripotent Stem Cell 5.2 Cellular and gene therapies Liver Disease Cell Differentiation 5.9 Resources and infrastructure Microfluidic Analytical Techniques Biological Sciences Stem Cell Research 5.9 Resources and infrastructure (treatment development) Drug Combinations Orphan Drug Good Health and Well Being 5.1 Pharmaceuticals Hepatocytes Proteoglycans Collagen Laminin Generic health relevance Development of treatments and therapeutic interventions Digestive Diseases Cardiac Biotechnology |
| Zdroj: | Stem cell research & therapy, vol 4 Suppl 1, iss Suppl 1 Stem cell research & therapy, vol 4 Suppl 1, iss SUPPL.1 Mathur, A; Loskill, P; Hong, S; Lee, J; Marcus, SG; Dumont, L; et al.(2013). Human induced pluripotent stem cell-based microphysiological tissue models of myocardium and liver for drug development.. Stem cell research & therapy, 4 Suppl 1. doi: 10.1186/scrt375. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/6p27g0xx |
| DOI: | 10.1186/scrt375. |
| Popis: | Drug discovery and development to date has relied on animal models, which are useful but are often expensive, slow, and fail to mimic human physiology. The discovery of human induced pluripotent stem (iPS) cells has led to the emergence of a new paradigm of drug screening using human and disease-specific organ-like cultures in a dish. Although classical static culture systems are useful for initial screening and toxicity testing, they lack the organization of differentiated iPS cells into microphysiological, organ-like structures deemed necessary for high-content analysis of candidate drugs. One promising approach to produce these organ-like structures is the use of advanced microfluidic systems, which can simulate tissue structure and function at a micron level, and can provide high-throughput testing of different compounds for therapeutic and diagnostic applications. Here, we provide a brief outline on the different approaches, which have been used to engineer in vitro tissue constructs of iPS cell-based myocardium and liver functions on chip. Combining these techniques with iPS cell biology has the potential of reducing the dependence on animal studies for drug toxicity and efficacy screening. |
| Databáze: | OpenAIRE |
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