Somatic embryos formation in Glycine max, Incasoy-27 variety
| Autor: | Pérez-Pérez, Jorge L., García, Lourdes R., Novisel Veitía, Idalmis Bermúdez-Carballoso, Raúl Collado López |
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| Zdroj: | BASE-Bielefeld Academic Search Engine Biotecnología Vegetal, Vol 9, Iss 4 (2009) |
| Popis: | The soybean crop is a major source of vegetable oil and protein concentrate worldwide. The tissue culture techniques are a tool for genetic improvement in soybean, however, established methodologies have low efficiency and are specific to certain genotypes but not for Cuban varieties. Then, it is necessary to establish a methodology for plant regeneration via somatic embryogenesis as a basis for genetic transformation programs. The aim of this study was to obtain embryos in variety Incasoy-27 from immature cotyledons. The effect of immature cotyledon size was determined and morphology of somatic embryos was evaluated. The embryos were obtained in MSD40 culture medium containing Murashige and Skoog salts, 40mg.l-1 2,4-dichlorophenoxyacetic acid, vitamin B5, 3.0% sucrose, pH 7.0, and Gelrite 0.3%, from immature cotyledons of 2.0 to 4.0 mm long, in artificial light conditions. Embryos formation in soybean variety Incasoy-27 was achieved by direct somatic embryogenesis and with a high frequency in all treatments. The 77% of cotyledons of three to four millimetres formed somatic embryos. Key words: Somatic embryogenesis,Soybean,Tissue culture |
| Databáze: | OpenAIRE |
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