Validation and harmonization of diagnostic methods for the detection of Grapevine Pinot gris virus (GPGV)
| Autor: | Gentili, A., Angelini, E., Babini, A.R., Bertazzon, N., Bianchi, G.L., Botti, S., Calvi, M., Cardoni, M., Casati, P., Cosmi, T., Gambino, G., Gualandri, V., Kubaa, R.A., Malossini, U., Martini, M., Mason, M., Raiola, A., Ratti, C., Saldarelli, P., Silletti, M.R., Tarquini, G., Faggioli, F. |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | 19th Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG), pp. 38–39, Santiago (Chile), 09-12/04/2018 info:cnr-pdr/source/autori:Gentili A., Angelini E., Babini A.R., Bertazzon N., Bianchi G.L., Botti S., Calvi M., Cardoni M., Casati P., Cosmi T., Gambino G., Gualandri V., Abou Kubaa R., Malossini U., Martini M., Mason G., Raiola A., Ratti C., Saldarelli P., Silletti M.R., Tarquini G., Faggioli F./congresso_nome:19th Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG)/congresso_luogo:Santiago (Chile)/congresso_data:09-12%2F04%2F2018/anno:2018/pagina_da:38/pagina_a:39/intervallo_pagine:38–39 |
| Popis: | Grapevine pinot gris virus (GPGV) has been originally found in Northern Italy associated to symptoms of stunting, chlorotic mottling and leaf deformations in grapevine plants. Successively, its presence has been reported in many countries even on asymptomatic plants. The study of this virus with regard to its biological and molecular characterization, its etiological role and spread by Eryophid mites vectors Colomerus vitis, the symptoms on different grapevine varieties cannot disregard the use of reliable, robust and validated diagnostic methods to implement possible prevention strategies. To this aim, a test performance study (TPS) was carried out to evaluate, harmonize and validate four diagnostic protocols used in the detection of GPGV in symptomatic and asymptomatic grapevine material. Specifically, two end point RT-PCR (Glasa et.al. 2014 - protocol 1; Saldarelli et al. 2015 - protocol 2) and two real time RT-PCR (Bianchi et al. 2015 - protocol 3; Ratti personal communication 2015- protocol 4) protocols have been compared. Obtained performance data from the four molecular protocols are reported and evaluated in the present Abstract. Both diagnostic methods, (end point and real time RT-PCR) were proven to be highly effective, although analytical sensitivity was more efficient for real time protocols, as expected. No statistically significant differences were observed for the other validation parameters. Moreover, all protocols gave satisfactory results even if six samples with low virus concentration were selected to stress the tests. Despite the large number of laboratories that performed the TPS and the different instruments and extraction methods (Nitrogen or different mechanic grinding system), no statistically significant differences were observed in the analytical specificity using the non target samples infected by other viruses than GPGV. All protocols showed good accuracy and robustness as proven using a large number of samples in a high number of laboratories. In conclusion, harmonized and validated reference protocols for the diagnosis of GPGV are, for the first time, available. |
| Databáze: | OpenAIRE |
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