Micro magnetic resonance imaging of murine liver tissue slices on a microfluidic perfusion device
| Autor: | Manvendra Sharma, Bishnubrata Patra, Hale Iii, William W., Ruby Karsten, Gert Salentijn, Maciej Grajewski, Erwin Fuhrer, Anna Zakhurdaeva, Dario Mager, Jan Korvink, Peter Olinga, Elisabeth Verpoorte, Marcel Utz |
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| Přispěvatelé: | Pharmaceutical Analysis, Pharmaceutical Technology and Biopharmacy, Medicinal Chemistry and Bioanalysis (MCB) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 Scopus-Elsevier 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society University of Groningen 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, 1722-1724 STARTPAGE=1722;ENDPAGE=1724;TITLE=22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
| Popis: | Micro-MRI (Magnetic Resonance Imaging) and NMR (Nuclear Magnetic Resonance) spectroscopy are used to image and study metabolic activity of precision-cut liver slices (PCLS) on a microfluidic perfusion device. The imaging experiments were performed under static medium conditions while metabolic activity was monitored under continuous flow of medium. LDH (Lactate dehydrogenase) leakage assay was performed to assess the tissue viability on different days. MR images having ~30 (μm)2 in-plane resolution are recorded. This method could be used to culture and monitor PCLS non-invasively. |
| Databáze: | OpenAIRE |
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