Three BUB1 and BUBR1/MAD3-related spindle assembly checkpoint proteins are required for accurate mitosis in Arabidopsis

Autor: Paganelli, Laetitia, Caillaud, Marie-Cécile, Quentin, Michael, Damiani, Isabelle, govetto, Benjamin, Lecomte, Philippe, Karpov, Pavel A, Abad, Pierre, Chabouté, Marie Edith, Favery, Bruno
Přispěvatelé: Institut Sophia Agrobiotech (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), UMR 7254, Centre National de la Recherche Scientifique (CNRS), The Sainsbury Laboratory [Norwich] (TSL), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine (NASU), UR 2357 conventionné avec l'Université de Strasbourg, INRA, French Government (National Research Agency, ANR) ANR-08-GENM-014 ANR-11-LABX-0028-01, Ministere de la Recherche et l'Enseignement Superieure, Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), The Sainsbury Laboratory, John Innes Centre [Norwich], Institut de Biologie Moléculaire des Plantes (IBMP)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: New Phytologist
New Phytologist, 2015, 205 (1), pp.202-215. ⟨10.1111/nph.13073⟩
New Phytologist, Wiley, 2015, 205 (1), pp.202-215. ⟨10.1111/nph.13073⟩
New Phytologist, Wiley, 2015, 205, pp.202-215
ISSN: 0028-646X
1469-8137
DOI: 10.1111/nph.13073⟩
Popis: The above article, first published online on Wiley Online Library (wileyonlinelibrary.com), and in New Phytologist 205: 202–215, has been retracted by agreement between the authors, the journal Editor-in-Chief, Alistair Hetherington, and John Wiley & Sons Ltd. Since publication of the above article, it has been brought to our attention that errors occurred in the construction of Figs 1 and 2(a); some components were inappropriately edited and duplicated, including the duplication and editing of images that first appeared in Caillaud et al. (2009), which were used by the authors as a basic template. Consequently, the integrity of the yeast two-hybrid experiments reported in the article is undermined, and, with agreement of all parties, the decision has been made to retract this article. We apologize for any inconvenience the publication of this work may have caused our readers. See : https://doi.org/10.1111/nph.14225; The spindle assembly checkpoint (SAC) is a refined surveillance mechanism which ensures that chromosomes undergoing mitosis do not segregate until they are properly attached to the spindle microtubules (MT). The SAC has been extensively studied in metazoans and yeast, but little is known about its role in plants. We identified proteins interacting with a MT-associated protein MAP65-3, which plays a critical role in organising mitotic MT arrays, and carried out a functional analysis of previously and newly identified SAC components. We show that Arabidopsis SAC proteins BUB3.1, MAD2, BUBR1/MAD3s and BRK1 interact with each other and with MAP65-3. We found that two BUBR1/MAD3s interacted specifically at centromeres. When stably expressed in Arabidopsis, BRK1 localised to the kinetochores during all stages of the mitotic cell cycle. Early in mitosis, BUB3.1 and BUBR1/MAD3.1 localise to the mitotic spindle, where MAP65-3 organises spindle MTs. A double-knockout mad3.1 mad3.2 mutant presented spindle MT abnormalities, chromosome misalignments on the metaphase plate and the production of lagging chromosomes and micronuclei during mitosis. We conclude that BRK1 and BUBR1/MAD3-related proteins play a key role in ensuring faithful chromosome segregation during mitosis and that their interaction with MAP65-3 may be important for the regulation of MT-chromosome attachment.
Databáze: OpenAIRE
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