Dynamics of production of MIP-1 alpha, MCP-1 and MIP-2 and potential role of neutralization of these chemokines in the regulation of immune responses during experimental autoimmune neuritis in Lewis rats
| Autor: | Zou, Lp, Pelidou, Sh, Abbas, N., Deretzi, G., Mix, E., Marianne Schultzberg, Winblad, B., Zhu, J. |
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| Předmět: |
Male
Monokines/*biosynthesis/immunology Chemokine CXCL2 Freund's Adjuvant Chemokine CCL2/*biosynthesis/immunology Sciatic Nerve/immunology/metabolism/pathology T-Lymphocytes/immunology Antibodies Rats Neutralization Tests Rats Inbred Lew Disease Progression Animals Immunization Macrophage Inflammatory Proteins/*biosynthesis/immunology Macrophages/chemistry/immunology Chemokine CCL4 Neuritis Autoimmune Experimental/*immunology/pathology Polyradiculoneuropathy/immunology Chemokine CCL3 |
| Zdroj: | Karolinska Institutet |
| Popis: | Experimental autoimmune neuritis (EAN) is an inflammatory autoimmune demyelinating disease of the peripheral nervous system (PNS) and represents an animal model of Guillain-Barre syndrome (GBS), which is a major inflammatory demyelinating disease of the PNS in humans. In the present study, the dynamics of the expression of the chemokines macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-2 and monocyte chemotactic protein-1 (MCP-1) were determined in the sciatic nerves of EAN rats. Additionally, the effect of neutralizing antibodies against MIP-1alpha, MIP-2 and MCP-1 on the clinical course of EAN and the chemokine expression was investigated. The maximum of MIP-1alpha positive cells in the sciatic nerves was seen on day 14 post immunization (p.i.) correlating with the development of severe clinical signs. Administration of an anti-MIP-1alpha antibody suppressed the clinical signs of EAN and inhibited inflammation and demyelination in the sciatic nerve. Peak numbers of MCP-1 positive cells in the sciatic nerves were detected on day 7 p.i. Administration of an anti-MCP-1 antibody caused a delay of onset of EAN. However, 4 of the 6 EAN rats receiving the anti-MCP-antibody showed the same degree of inflammatory cell infiltration and demyelination in the sciatic nerves as sham-treated EAN rats, whereas only 2 EAN rats had less inflammation and demyelination. The numbers of MIP-2 positive cells reached a maximum on day 21 p.i. Anti-MIP-2 antibody failed to suppress the clinical signs of EAN and the inflammation and demyelination in the sciatic nerves. Only administration of the anti-MIP-1alpha antibody resulted in a significant reduction in the number of chemokine (MIP-1alpha)-positive cells and ED1-positive macrophages in the sciatic nerves. The present results demonstrate that MIP-1alpha and MCP-1 may play a role in the immunopathogenesis of EAN, and that MIP-1alpha induced trafficking of inflammatory cells can be inhibited by immunoneutralization. Further elucidation of the regulation and coordination of MIP-1alpha and MCP-1 production may lead to new therapeutic approaches to GBS in humans. J Neuroimmunol |
| Databáze: | OpenAIRE |
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